Study On The DNA Methylation Modification Of The Susceptible Gene In Patients Of Primary Type 2 Diabetes In Uygur

Objective:To investigate the level of DNA methylation modification in the target area gene of primary type 2 diabetes(T2DM)in Uygur.Methods:5 primary T2 DM patients and 5 control subjects were selected.DNA was extracted from peripheral blood leukocytes,DNA methylation level of susceptible genes were detected by bisulfite sequencing(BSP).Compared the difference of DNA methylation level between cases and controls.Result:(1)The CpG sites methylation levels in functional genomic regions,the highest DNA methylation rate were in 3’untranslated region and repeat region,the lowest DNA methylation rate were in promoter area,5’untranslated region and CPG island,the DNA methylation in the functional areas was lower than the control group,except for CPG island coastal area.(2)Compared with the control group,there was difference in the level of DNA methylation in the different methylation sites(DMC).The DNA methylation level in the cases was lower than the controls in the differential methylation area(DMR).(3)Based on the distribution of DMR in the genome,the analysis of functional enrichment was found:GO functional analysis shows that the hypomethylation gene is mainly involved in the molecular function,and the hypermethylation gene participates in the molecular function and biological process.Analysis of KEGG signal pathway,it was mainly the ribosome signaling pathway.Conclusion: Analysis the DNA methylation in the target area of the peripheral blood sample in the primary T2 DM was founded:There was difference in DNA methylation level between cases and controls,functional enrichment analysis showed that the differential methylation gene mainly participates in molecular function and focuses on ribosomal signaling pathway.This study has preliminarily found that epigenetic modification may be involved in the development of T2 DM.