Relationship Between Interleukin 22(IL-22)and Liver Fibrosis Of Hepatitis B And Its Clinical Diagnostic Value

BackgroundHepatitis B is one of the most common infectious diseases,and is a high incidence area of HBV infection in our country.Hepatitis B infection and liver cirrhosis bring huge economic burden to patients and society.Liver fibrosis,however,is an early stage and an essential stage of all cirrhosis and can be reversed in certain circumstances.Histopathological examination is the gold standard for the diagnosis of hepatic fibrosis,but it is a invasive examination.Transient elastography Fibro Scan diagnostic efficacy is increasingly prominent,and serological models APRI score and the FIB-4 index are also recommended by a number of guidelines for the diagnosis of hepatic fibrosis.is the activation of HSC(hepatic stellate cell)as the center.A variety of cytokines play a key role in the pathogenesis of hepatic fibrosis[1].Studies at home and abroad suggest that IL-22 is related to liver fibrosis.Transforming growth factor(transforming,growth,factor-β1 TGF-β1)is recognized as the most critical cytokine in liver fibrosis.The purpose of this study was to investigate the relationship between cytokine interleukin-22(IL-22)and hepatitis B liver fibrosis and its clinical diagnostic value.ObjectiveTo discuss the correlation between IL-22 and TGF-β1.The changes of IL-22 levels in different stages of hepatic fibrosis,and the correlation between IL-22 and hepatic fibrosis were analyzed.Comparison of the diagnostic value of IL-22,APRI,FIB-4 and index combination in different hepatitis B liver fibrosis groups,and to analyze the value of IL-22 in the staging of liver fibrosis.MethodsFrom May 2016 to June 2017 deadline,in Fist Affiliated hospital of Xinxiang Medical University,liver biopsy,Fibro Scan and laboratory examination of chronic hepatitis B patients were collected.In the past year,no antiviral,anti-fibrosis and immune modulators have been used,and other immune system diseases and other viral infections have not been incorporated,and informed consent has been obtained.CHB patients were divided into five groups according to the scan of the to the pathological tissue stage,ten people belonged to FO group,twenty one people belonged to F1 group,fourteen people belonged toF2 group,ten people belonged to F3 group,eight people belonged to F4 group.There were no obvious hepatic fibrosis group(FO group,Fl group)thirty-one people,and there were thirty-two people in the liver fibrosis group(be equal or greater than F2 group),and eight people in the early cirrhosis group(F4 group).The ELISA in serum of patients with each group of IL-22,TGF-β1,using conventional automatic biochemical analyzer determination,AST,PLT,ALT and TBIL,calculate APRI,FIB-4 index.Results1、The correlation between IL-22 and TGF-β1:r = 0.850,P = 0.000,IL-22 related to TGF-β1.2、The clinical data of no obvious hepatic fibrosis group,obvious hepatic fibrosis group and early cirrhosis group.were compared.The correlation between IL-22 and hepatic fibrosis staging was r =-0.635,P value = 0.000,and IL-22 was statistically significant between groups(F = 24.21 P = 0.00).The difference of IL-22 in the absence of obvious hepatic fiber group was statistically significant in the early cirrhosis group.There was no statistically significant difference between hepatic fibrosis and early cirrhosis.IL-22 is related to hepatic fibrosis staging.3、The AUC values of IL-22 diagnosis with or without obvious hepatic fibrosis were 0.883,respectively,and the P values were less than 0.05,which was statistically significant.The AUC values of IL-22 diagnosis with or without early cirrhosis were 0.723,respectively,and the P values were less than 0.05,which were statistically significant.In general,the diagnostic value of IL-22 is high.4.The AUC values of IL-22 + APRI,IL-22 + FIB-4,IL-22 + APRI + FIB-4 diagnosis with or without obvious hepatic fibrosis were 0.972、0.974、0.975,P values were less than 0.05,there was statistical significance.The AUC values of IL-22 + APRI,IL-22 + FIB-4,IL-22 + APRI + FIB-4 diagnosis with or without early cirrhosis were 0.793、0.800、0.805,P values were less than 0.05,there was statistical significance.The combination of IL-22 and other indexes can significantly improve the diagnostic efficacy.ConclusionIL-22 is negatively correlated with liver fibrosis.IL-22 has a certain diagnostic value in diagnosis of obvious hepatic fibrosis and early cirrhosis.Combined with APRI,FIB-4 can improve the diagnostic ability.