| Different ethnic groups have different dietary culture and different geographical characteristics,different living habits,so created some of the disease manifested in different ethnic and geographical differences,and osteoporosis disease as a chronic aging disease,the current trend was Especially in the postmenopausal women with postmenopausal osteoporosis was significantly more than men,at home and abroad on different ethnic groups,different regions of bone mineral density and vitamin D levels of polymorphism and different ethnic diet,exercise,age,and menopause Age and other related research done and found that some different ethnic and regional levels of vitamin D polymorphism and bone density and age,eating habits,sports and other differences,Ningxia as the Hui Autonomous Region,Hui has a unique diet and cultural habits and Different ethnic customs,so the subject from the Han nationality of different ethnic menopausal women to carry out,to study the two ethnic groups of the Chinese people’s vitamin level polymorphism and syndromes associated with TCM.This experiment mainly from two aspects to do1.Analysis of General Information and Bone Mineral Density and TCM Syndromes of Collecting and Screening Menopausal Women in2.The relationship between VDR receptor gene polymorphism and bone mineral density and TCM syndromes was studied in the above screening data.Objective1.To explore the correlation between the level of vitamin D receptor gene polymorphism and bone mineral density and TCM syndromes in postmenopausal women of different ethnic groups in Hui nationality.2.To explore the correlation between the level of vitamin D receptor gene polymorphism and bone mineral density and TCM syndromes in postmenopausal women of different ethnic groups in Hui nationality.MethodsSelected Ningxia Medical University Affiliated General Hospital outpatient and physical examination of patients,the time in July 2015 to 20 cases of postmenopausal women who returned to China in May 2016 were the subjects,60 cases were returned to Han,and randomized,single blind And the control of the experimental design method,the patients were based on bone mineral density values are divided into four groups,divided into the normal group of Hui,Hui Osteoporosis group,Han normal group,Han group of osteoporosis group4,each group of 30 cases,and further investigation Questionnaires,asked about their eating habits,exercise,menopause age and other information,and asked the deputy chief physician above based on patient disease,An Analysis of Traditional Chinese Medicine Syndromes.And compare the different bone mineral density and TCM syndromes of different ethnic groups.In Ning Xia Medical University General Hospital outpatient and physical examination Office to meet the needs of the sample of menopausal women,back to the Han 60 cases,and fill out the clinical questionnaire,measuring L2-L4 bone mineral density values,and take the mean,analysis of TCM syndromes,complete the blood And the gene polymorphism was analyzed by PCR,electrophoresis and gene sequencing.The genetic polymorphisms of different ethnic groups were analyzed and analyzed.Correlation between bone mineral density and TCM syndromes.Results1.There was no significant difference in bone mineral density between the normal groupand the loose group.2.There was no significant difference between bone mineral density and TCM syndromes in normal menopausal women.Χ2 value of 0.230,P = 0.973> 0.05;back to menopausal women loose group of patients with bone mineral density and TCM syndromes were no significant difference.Χ2 value of 0.825,P = 0.843> 0.05,according to ɑ = 0.05 level.3.The frequency of VDR Fokl allele was 63.3% and 36.6% in the normal menopausal female of Yin chuan Han nationality,and the frequency of gene group was 55.0% and 45.0%,and the value of χ2 was 1.226,P = 0 <0.05,There were differences in the allele between the normal group and the loose group,indicating that the number of alleles(C,T)carried by the Han group was different from that of the normal group.4.There are differences in the Fokl gene of vitamin D receptor in postmenopausal women in Yin chuan area.P = 0 <0.05.5.The bone mineral density of Fokl locus SNP gene type in VDR receptor gene polymorphism in postmenopausal women in Yinchuan area was significantly higher than that in Han menopausal women and Hui menopausal women(P = 0.009 <0.05)CC gene type of the Hui menopausal women with high bone mineral density than the Han menopause.6.The results showed that there was no significant difference between TCM gene type and vitamin D receptor gene type in the postmenopausal women in Yinchuan area(χ2 = 5.931,P= 0.052> 0.05).(P <0.05),indicating that there was no significant correlation between the osteoporosis patients and the vitamin D receptor gene in the postmenopausal women of the Han nationality,and there was no significant correlation between the Han group and the Hui nationality group,In the other three groups can be seen in the correlation,indicating that some genes or carry the different genes caused by this is worthy of our later to explore a problem.Conclusions1.Back to the Han and shen-yang were accounted for(37%、30%),followed by liver and kidney yin deficiency,respectively,23%,27%,spleen and kidney yang and qi stagnation type rare,from which you can see the bone patients with loose kidney to kidney type is themost common.2.This study shows that there is no significant difference between bone mineral density and TCM syndromes in normal menopausal women.Χ2 value of 0.230,P = 0.973> 0.05;back to menopausal women loose group of patients with bone mineral density and TCM syndromes were no significant difference.Χ2 value of 0.825,P = 0.843> 0.05,according to ɑ = 0.05 level.It is thought that there is no significant difference in the postmenopausal women in the postmenopausal bone mineral density level,which may be related to the small sample size,or the error in the survey statistics,or the residence of the Hui nationality is long and the living habits are similar.3.The bone mineral density of Fokl locus SNP gene type in VDR receptor gene polymorphism in postmenopausal women in Yin chuan Hui-Han region was significantly higher than that in postmenopausal women and postmenopausal women with postmenopausal women(P = 0.009 <0.05)CC gene type of postmenopausal women with bone mineral density than the Han postmenopausal women.4.There was no significant correlation between bone mineral density and TCM syndromes in postmenopausal women in Yin chuan area,and it was considered that their different lifestyles and living conditions were different or had different heredity.5.There was no significant difference between the normal group and the bone mineral density of the postmenopausal women in Yin chuan area.6.The results showed that there was no significant difference between TCM gene type and vitamin D receptor gene type in the postmenopausal women in Yin chuan area(χ2 = 5.931,P= 0.052> 0.05).(P <0.05),indicating that there was no significant correlation between the osteoporosis patients and the vitamin D receptor gene in the postmenopausal women of the Han nationality,and there was no significant correlation between the Han group and the Hui nationality group,In the other three groups can be seen in the correlation,indicating that some genes or carry the different genes caused by this is worthy of our later to explore aproblem.7.The alleles of the vitamin D receptor gene between the normal group and the loose group were different in Yinchuan area.In this study,the sample size is small and the representation range is small,but it can be used for the future study of large sample data.It can make corresponding contribution to the development and prevention of ethnic minorities.By increasing the sample size,It is important for us to study the differences of pathogenesis,genetic factors and prevention and treatment of osteoporosis in ethnic minorities and Han nationality. |
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