SFRP5 Silencing Promotes Malignant Phenotype Of Human Pancreatic Cancer Cell PANC-1 And CFPAC-1

Objective: To investigate the effect of lentivirus-mediated shRNA silencing SFRP5 on malignant phenotype of pancreatic cancer cell PANC-1 and CFPAC-1.Methods: A SFRP5-knockdown recombinant plasmid was successfully transfected into pancreatic cancer cell line PANC-1 and CFPAC-1,blank plasmid was transfected into two group of cells as negative control and untreated cells as blank control group.The transfection efficiency of the experimental group and the negative control group was observed under the fluorescence inverted microscope,the expression of SFRP5 at RNA and protein level in cell were detected by real-time PCR and western blot,CCK-8 assay was used to detect the proliferation of pancreatic cancer cells,the cell migration of pancreatic cancer cell line PANC-1 and CFPAC-1 was analyzed by Transwell migration assay and cell scratch test.Results: The transfection efficiency of the experimental group and negative control group was more than 80% under inverted fluorescence microscope,and no significant decrease in the expression of green fluorescent protein expression was observed 48 hours later,the results of real-time PCR and western blot showed that stable transfected shRNA-SFRP5 cell of pancreatic cancer lines PANC-1 and CFPAC-1 were established successfully.The proliferation capacity of SFRP5 shRNA group was significantly higher compared to the negative control and blank control group by CCK8 assay(P<0.01),there is no significant difference between the negative control group and blank control group.Similarly,the results of Transwell assay and cell scratch test showed that cell migration of SFRP5 shRNA group was significantly higher compared to the negative control and blank control group(P<0.01),there is no significant difference between the negative control group and blank control group.Conclusions: SFRP5-shRNA lentiviral interference vectors can effectively decrease the expression of SFRP5 gene in PANC-1 and CFPAC-1 cell of pancreatic cancer,SFRP5 can significantly inhibit the proliferation,invasion and metastasis of pancreatic cancer cells.