The Drug Sensitivity Assessment Of Prostate And Pancreatic Cancer Cells By Real-time Cell Analysis Inhibitory Effect Of Low Expression Of Trim46 Gene On Proliferation And Migration Of Pancreatic Cancer Cell Line Capan-2

Objective:To explore the sensitivity of different tumor cell lines to drugs based on the development of real-time cell analysis and to provide a more simple and stable method to provide reference for clinical treatment of prostate cancer and pancreatic cancer.Method:Five human prostate cancer cell lines VCaP、DU145、PC-3、PC-3M-2B4 and PC-3M-IE8 and Three human pancreatic cancer cell lines SW1900、Capan-2、PANC-1 were selected.Selection of docetaxel,carbapine and acetic acid abirater three kinds of prostate cancer commonly used drugs,and gemcitabine hydrochloride and tiggio capsule two common treatment of pancreatic cancer drugs.Cells were incubated for 24 hours and then incubated at different concentrations.RTCA was used to monitor the cell growth curve before and after administration.The semi-inhibitory concentration(IC50)of drugs on prostate cancer cells and pancreatic cancer cells was calculated.The IC50 of Gemcitabine Hydrochloride and Tiggio Capsules was validated by AO/EB staining and laser confocal observation.Result:The IC50 of docetaxel to VCaP,DU145,PC-3,PC-3M-2B4 and PC-3M-IE8 were 8.81 nM,11.61 nM,1.78 nM,1.44 nM and 8.69 nM,respectively.The IC50 of carbachazate for the 24 h cell lines was 3.73 nM,3.96 nM,10.41 nM,5.43 nM,7.37 nM,respectively.The IC50 of the five cell lines at 24 hours was 8.34μM,8.60μM,24.20μM,8.59μM,13.21μM,respectively.The IC50 of gemcitabine hydrochloride on SW1900,Cpan-2 and PANC-1 cells was 1.69μmol/L,10.05μmol/L and 12.74μmol/L,respectively.The IC50 of the three cell lines was 180.29μmol/L,765.70μmol/L and 95.57μmol/L,respectively.AO/EB staining verify IC50 is true and reliable.Conclusion:PC-3M-2B4 and DU145 can be used as control of docetaxel;VCaP and PC-3 can be used as control of carbapine and acetic acid albitron,SW1990 and Capan-2 can be used as gemcitabine hydrochloride and tiggio The establishment of cell model,the drug in vitro sensitivity screening for clinical application to provide a reference.Objective:To investigate the effect of low expression of Trim46 on the proliferation and migration ability of pancreatic cancer cell line Capan-2,the expression of Trim46 in Capan-2 cells and the relationship between the expression of tumor-associated proteins and its expression level were observed.Methods:Construction of Trim46 low expression of Capan-2 cells using miR RNAi silencing.The expression of Trim46 in Capan-2 cells was detected by Western blot and immunofluorescence.The expression of Trim46 was detected by immunofluorescence.The co-localization of Trim46 in Capan-2 cells was detected by immunofluorescence The effect of low expression of Trim46 on the expression of Tumor protein in Capan-2 cells was detected by Western blot.The effect of low expression of Trim 46 on the proliferation of Capan-2 cells was detected by RTCA.The effect of low expression of Trim46 on migration ability of Capan-2 cells was investigated by wound healing experiment.Results:Trim46 was expressed in the cytoplasm and cell membrane of Capan-2,which was a point or filamentous structure.Trim46 was co-locating with actin,fak and integrin beta2、integrin beta6,and Trim46 low expression The expression of fibrin-FAK pTyr861 was up-regulated,Cortactin(cortical actin)was down-regulated,ARP2(actin-associated protein)was up-regulated and CrKL was up-regulated;Trim46 was down-regulated can inhibit the proliferation and migration of Capan-2 cells.Conclusions:Trim46 gene may be related to tumor proliferation and migration ability.