Experimental Study Of Expression Of AQP3in Pancreatic Cancer And It’s Proliferation And Migration Ability Of PANC-1Cell Induced By EFG

Objective:The aim of this study was to investigate whether AQP3expression in the human pancreatic cancer cell lines, PANC-1,enhances cell migration. In this study we investigated whether AQP3is expressed in cultured PANC-1pancreatic cancer cells, and whether EGF induces AQP3expression via ERK signal transduction pathways and further more enhances cell migration.Methods:The expression of AQP3in mRNA level was detected in pancreatic cancer cell lines PANC-1and pancreatic cancer cell line PANC-1by RT-PCR. We screened the expression profile of AQP3in pancreatic cancer tissues and corresponding normal mucosa from80patients with pancreatic cancer by RT-PCR, western blot analysis, immunochemical assay and immunofluorescence. The relationship between AQP3expression and clinicopathologic characteristics of patients was evaluated. Cultured PANC-1cells were treated with epidermal growth factor (EGF) and subjected to cell migration assay. The expression or activation level of proteins was analyzed by western blot.Results:Here, we showed that AQP3is expressed in the human pancreatic cancer cell lines PANC-1. The EGF induced AQP3expression in a time-and dose-dependent manner and increased pancreatic cancer cell migration. Further more, a mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) inhibitor U0126inhibited EGF-induced AQP3expression and cell migration.Conclusions:AQP3plays a critical role in EGF-induced cancer cell migration and that EGF induces AQP3expression via ERK signal transduction pathways.These finds provide evidence for a novel role of AQP3in pancreatic cancer as a potentially important determinant of tumor growth and spread. EGFR/ERK pathway mediated AQP3activation and cell migration stimulated by EGF in cultured PANC-1pancreatic cancer cell in vitro, and this cell signaling pathway can be inhibited by EGFR inhibitor and ERK inhibitor, which may be used as potential therapeutic targets in the treatment of pancreatic cancer.