Effect Of Jianpi Yiqi Decoction On ?-catenin And GSK3? Expression And Its Inhibitory Effect On CAG Rat Model

Objective:To observe the effect of Jianpi Yiqi Decoction in the process of chronic atrophic gastritis?CAG?model rats that the serum levels of PGE2,GAS,SS and the expression of gastric mucosa tissues beta-catenin and GSK-3beta,so as to explore the preventive effect and mechanism of Jianpi Yiqi Decoction on the incidence of chronic atrophic rats of the disease.Methods:35 SD male rats as the experimental rats that were randomly divided into normal group?normal feeding?,model group?0.9%Nacl?,Jianpiyiqi Decoction low dose(13.14g.kg^-1.d^-1),middle dose(26.28g.kg^-1.d^-1)and high dose(52.56g.kg^-1.d^-1)group,each group of seven,the model group using MNNG with ranitidine preparation CAG large Rats,Jianpiyiqi Decoction of different dosage groups in the model at the same time giving the intervention of traditional Chinese medicine,1 times daily,2ml each time,All rats were sacrificed at the end of 24th week,To observe the general conditions of rats,gastric mucosal pathology,the content changes of expression using Elisa kit to detect the PGE2,GAS,SS;The expression of ?-catenin and GSK3(3 in gastric mucosa were measured by immunohistochemistry.Results:?The general condition:The rats in the normal group were in good mental state,responsive,the hair was white and shiny,the normal eating and the weight gain were fast.The rats in the model group were sparsely populated with poor activity,Decreased weight and slow weight growth;traditional Chinese medicine in different doses of mental state,diet and weight,etc.Close to the normal group.?The morphology:normal group of gastric mucosal reddish,fold,mucous membrane,soft and elastic;Model group rats with gastric mucosa are pale,fold to reduce in the normal group and the small,mucosal wall thinning,elasticity,surface erosion and dotted hemorrhage stove,part of the rat gastric mucosa wall rough stiffness,have of polyps.Traditional Chinese medicine?TCM?doses of intervention group grossly gastric mucosal morphology was close to normal.?The pathological histology changes:normal gastric mucosa structure level complete set,closely packed glands and rules;Model group rats mucosal layer thinning,arrangement of irregular glands,atrophy,inflammatory cells infiltration;See glands are arranged in the lower dosage treatment groups compared with normal group is irregular,inflammatory cells infiltration,did not see mucous membrane thinning;high dose of intervention group in gastric mucosa structure integrity,glandular arrangement rule,close to the normal group.?Changes of Serum PGE2,GAS and SS contents in Rats:The levels of PGE2,GAS and SS in the model group were significantly lower than those in the normal group?P<0.01?.The patients with low,medium and high dose were significantly higher than those in the model group?P<0.05 and 0.01?There was no significant difference between the three groups?P>0.05?.?Expression of ?-catenin and GSK3? in Gastric Mucosa Tissue:Compared with the normal group,the expression of ?-catenin in the model group was significantly higher than that in the normal group?P<0.01?,and the expression of GSK-3? was significantly decreased?P<0.01??P<0.01?.There was no significant difference in the expression of ?-catenin and GSK3?between the two groups?P>0.05?.The expression of GSK3P in the middle and high dose groups was significantly decreased?P<0.05?.Conclusion:?Jianpi Yiqi Decoction can effectively improve the general condition of CAG rats,protect gastric mucosal barrier,promote gastric mucosal repair,and block the pathological changes of gastric mucosal atrophy in rats,but no obvious amount Relationship.?Preventive administration of spleen and qi recipe can regulate the changes of serum PGE2,GAS and SS in the pathogenesis of the model rats,so as to improve the gastric secretion function and help to prevent the occurrence,development and aggravation of CAG.?The expression of?-catenin in the gastric tissue during the pathogenesis of the model rats was increased,and the expression of GSK-3? was increased,which could promote the differentiation and apoptosis of gastric mucosal cells.That one of the molecular mechanisms.