| Objective By observing the impact of HuaZhuoJeDu Decoction on chronic atrophic gastritis in patients with clinical symptoms, serum motilin, gastrin, somatostatin levels and on chronic atrophic gastritis model rat's general situation, gastric mucosal morphology, motilin (MOT), gastrin (GAS), somatostatin (SS) level change, investigate the mechanism of HuaZhuoJeDu Decoction in treatment of chronic atrophic gastritis and lay the foundation for further study of HuaZhuoJeDu Decoction.Method 1. Clinical Study: From July 2008 to October 2008, 40 cases that qualify as eligible patients were selected, in Hebei Province Chinese Medicine Hospital gastroenterology wards. And 22 cases were divided into treatment group and control group, 18 patients, by random number method. HuaZhuoJeDu Decoction group was treated with HuaZhuoJeDu Decoction daily 300ml, morning and evening hours twice took. And Control group was given WeiFuChun, each 4, 3 times a day. The two groups were treated for three months. Compare the two groups before and after treatment in patients with clinical symptoms, and the level of MOT, GAS and SS.2. Experimental Study: 60 SPF level healthy 6-week-old Wistar male rats, in accordance with the principles of randomly divided into 2 groups, by random number method: The normal group 10 and build 50 modules. Rats of builded modules were made modules by integrated modeling method, total of 3 months. After the success of modeling, the 38 rats were divided into five groups by random number method: model group 8, WeiMeiSu group 7, HuaZhuoJeDu Decoction low dose group 7, HuaZhuoJeDu Decoction middle dose group 8, and HuaZhuoJeDu Decoction high dose group 8. And then begin to treat: low dose group use low dose HuaZhuoJeDu Decoction irritate rats stomach twice a day according to 5g/kg·d; Midddle dose group use middle dose HuaZhuoJeDu Decoction irritate rats stomach twice a day according to 10g/ kg·d; high dose group use high dose HuaZhuoJeDu Decoction irritate rats stomach twice a day according to 20g kg·d; The medicine were targeted to 4ml whith distilled water, and splited between the two. WeiMeiSu Group use suspending liquids of WeiMeiSu 4ml, splited between the two, Model group and normal group, irritate rats'stomach twce a day, with 4ml distilled water, splited between the two. All of the six group last 3 months. The rats were inhibited from drinking water for 24 hours after last administration. And all rats were killed to take blood sample and stomach after weighed. Detect MOT GAS SS by radioimmunoassay. And Observe general concept of gastric mucosa and gastric mucosal histopathology observed under light microscope. Process data with SPSS17.0, P<0.05 means the difference was significant. Date was expressed by x_±s, using t test, X2 test, and analysis of variance for statistical computing.Results 1. Clinical Study: (1)Two groups of patients with major syndromes improvement: treatment group 22 cases, the total effective rate was 95%.And control group patients 18, the total effective rate was 94%.Effects are significant differences in the two groups (P<0.05). (2)MOT: Comparison of the two groups before and after treatment, respectively, the results were statistically significant (265.643±89.620 pg/ml VS 290.240±105.589 pg/ml,239.491±87.786 pg/ml VS 259.885±97.011 pg/ml,P<0.01).And comparison of two groups before and after treatment changes, results no statistically significant (24.597±20.541 pg/ml VS 20.394±19.175 pg/ml,P>0.05). (3) GAS: Two results are statistically significant, Comparison of two groups before and after treatment and compared the two groups before and after treatment changes (86.833±28.620 pg/ml VS 95.383±28.976 pg/ml ,81.178±25.927 pg/ml VS 86.145±26.917 pg/ml,8.550±3.076 pg/ml VS 4.967±2.127 pg/ml,P<0.01). (4)SS: Two results are statistically significant, Comparison of two groups before and after treatment and compared the two groups before and after treatment changes (14.075±6.096 pg/ml VS 11.799±6.092 pg/ml, 12.287±6.393 pg/ml VS 10.800±6.605 pg/ml, 2.275±0.726 pg/ml VS 1.487±0.993 pg/ml,P<0.01).2. Experimental Study: (1) General condition of the rats: All the groups of treatment can improve rats'fur color, the state of mind, the appetite, and the activity. But the middle dose group and the high dose group had obvious influence. (2) Visual observation of gastric mucosa: Compared with model group, the gastric mucosal atrophy of rats, in Weimeisu group and HuaZhuoJeDu Decoction groups, had varying degree of improvement. But the middle dose group and the high dose group had obvious influence. (3) Light microscopic observation of gastric mucosa in rats: Compared with model group, the gastric mucosal atrophy and intestinal metaplasia of rats, in Weimeisu group and HuaZhuoJeDu Decoction groups, had varying degree of improvement. But the middle dose group and the high dose group had obvious influence. (4) MOT: model group (17.428±1.326pg/ml), Weimeisu group (21.942±1.663 pg/ml), low dose group (26.781±0.948 pg/ml), middle dose group (32.055±1.606 pg/ml) and high dose group (31.543±1.386 pg/ml) of serum MOT was significantly lower than the normal group (49.808±2.475 pg/ml) (P<0.05). And treatment groups of serum MOT was significantly higher than the model group (P<0.05), to middle and high dose group the most obvious. (5) GAS: model group (10.535±0.854pg/ml), Weimeisu group (13.035±0.522 pg/ml), low dose group (18.066±1.491 pg/ml), middle dose group (22.141±0.836 pg/ml) and high dose group (22.220±1.131 pg/ml) of serum GAS was significantly lower than the normal group27.004±1.625 pg/ml (P<0.05). And treatment groups of serum GAS was significantly higher than the model group (P<0.05), to middle and high dose group the most obvious. (6)SS: model group (19.846±1.231pg/ml), Weimeisu group (16.133±0.906 pg/ml), low dose group (13.366±0.801 pg/ml), middle dose group (11.053±0.976 pg/ml) and high dose group (10.907±1.232 pg/ml) of serum SS was significantly higher than normal group(5.035±0.699 pg/ml) (P<0.05). And treatment groups of serum GAS was significantly lower than the model group (P<0.05), to middle and high dose group the most obvious.Conclusions (1) HuaZhuoJeDu Decoction can improve clinical symptoms in patients with CAG. (2) HuaZhuoJeDu Decoction can improve the CAG of gastric mucosal injury. (3) HuaZhuoJeDu Decoction may by adjust the disorder of MOT, GAS and SS increase the nutritional role of the gastric mucosa and enhance mucosal barrier repair functions, to achieve the purpose of treatmentting chronic atrophic gastritis. (4) HuaZhuoJeDu Decoction treatment of CAG, when the side of each group, showing a certain dose-effect relationship, high-dose, medium-dose than low-dose effects of obvious, but high and middle dose was no significant difference between the effects of.
|
PDF Full Text Request