Effect Of Banxia Xiexin Decoction On Wnt/β-catenin Signaling Pathway In Rats With Chronic Atrophic Gastritis

Object:To explore the effect mechanism of Banxia Xiexin Decoction on the Wnt/β-catenin pathway in a rat model of chronic atrophic gastritis,and to compare the effect difference between different doses.Methods:The related pathways predicted by network pharmacology were validated by animal experiment design.The CAG model of spf-grade male Wistar rats was constructed by MNNG.After successful modeling,the modeling group was divided into model control group,banxia xiexin decoction high dose group,banxia xiexin decoction low dose group.by random number table method.Elisa was used to observe the changes of IL-1β,MTL,GAS,Wnt2,β-catenin,CyclinDl and c-myc in serum of each group.The protein expressions of Dvl,GSK3β and APC were detected by WesternBlot.The expressions of Wnt2,Dvl,APC,polyinin,CyclinD1 and c-myc in each group were observed by PCR.Results:Molecular biology experiments show that:①In terms of pathology,compared with banxia xiexin decoction low dose group,banxia xiexin decoction high dose group was more effective in reducing the infiltration of inflammatory cells and mucosal congestion in the gastric mucosa of chronic atrophic gastritis model,and had a better effect on the reduction and atrophy of gastric mucosa.②Elisa results showed that compared with the model group,the contents of IL-1β,GAS,Wnt2,β-catenin,Cyclin D1 and c-myc were significantly reduced in the banxia xiexin decoction high dose group(P<0.01)and increase the MTL content(P<0.01)。The content of IL-1β,gas,Wnt2,β-catenin,Cyclin D1 and c-myc in the low dose group of Banxiaxiexin Decoction were decreased(P<0.01)but MTL were increased.③Western blot results showed that compared with the model group,the high dose Banxiaxiexin Decoction group could effectively down regulate the expression of DVL(P<0.01),and up regulate APC(P<0.01),GSK3 β(P<0.05).At the same time,APC(P<0.05)and GSK3 β(P<0.05)were up-regulated.④On the regulation of Wnt/β-catenin signaling pathway,the results of PCR showed that compared with the model group,the expression of Wnt2 mRNA(P<0.05)、Dvl mRNA(P<0.05)、β-catinin mRNA(P<0.01)、CyclinD1 mRNA(P<0.05)and c-myc mRNA(P<0.05)could be down-regulated more effectively in the banxia xiexin decoction high dose group.Banxia xiexin Decoction low dose group can down regulate DVL mRNA(P<0.05),β-Catenin mRNA(P<0.05),and up regulate APC mRNA(P<0.05).Conclusion:Prediction of network pharmacology by animal experiments.The following conclusions are drawn from animal experiments:①Banxia xiexin decoction can reduce the inflammatory infiltration of gastric submucosa in the model of chronic atrophic gastritis,and effectively improve the reduction and atrophy of gastric mucosa.②Banxia Xiexin Decoction can inhibit the abnormal activation of Wnt/β-Catenin signal pathway,which may be one of the important mechanisms of Banxia Xiexin Decoction in the treatment of CAG.So it can inhibit the proliferation and accelerate the apoptosis of gastric epithelial cells and lamina propria cells,and prevent the mutation of gastric epithelial cells and lamina propria cells.③Compared with the model group,the high-dose group and the low-dose group of Banxia Xiexin Decoction can inhibit the abnormal activation of Wnt/β-Catenin signal pathway and improve the pathological changes,and the high-dose group is better than the low-dose group of Banxia Xiexin Decoction.