The Effect Of Endocan On The Malign Grade Of Gastric Adenocarcinoma Cell And Its Molecular Mechanism

Objective To investigate the effect of endocanon proliferation,migration and apo ptosis in human gastric cancer SGC7901 cells and potential molecular mechani sm.Methods the migration of SGC7901 cells were analyzed by Wound-healing ass ay.The apoptosis was observed by Hoechst 33,258 staining.The activities of alkaline phosphatase and lactate dehydrogenase were analyzed by using AKP and LDH kit s.Western blotting analysis was performed to examine the expression of migratio n and proliferation-associated proteins(MMP-9,E-Cadherin),apoptosis-associated pr oteins(caspase3,Bax,Bcl-2,p53).Results The wound-healing assay has shown,end ocan-OE cells showed lower migration ability while higher in si-endocan SGC7901 cells.The activities of AKP and LDH were both down in endocan-OE cells but w ere increased in si-endocan cells.The Hoechst 33,258 staining assay has shown,mo re apoptosis cells were observed in endocan-OE cells,and there wereless apoptosis cells in si-endocan cells.Western blot analysis showed that endocan had significa nt effect on suppressing the expression of MMP-9,Bcl-2 and theexpression of E-Cadherin,p53,caspase3,Bax were increased.Conclusion Endocan reduces the malign grade of gastric cancer cells by regulating associated protein expression.endocan could inhibit the migration of SGC7901 cells and promotes SGC7901 ce ll differentiation by regulating MMP-9,E-Cadherin expression.endocan enhance t he apoptosis in SGC7901 cells,which were regulated by p53,caspase3,Bax,Bc l-2.