The Significance Of Expression Of Endocan In Colorectal Cancer Tissue And Effect Of Butyrate On Expression Of Endocan In RKO Cell

Objective To investigate the expression regularity and allocation of endocan (human endocellular-specific molecule-1, ESM-1) in colon and rectum tissue; and analyze the variability of endocan expression between normal and cancerous colon and rectum tissue and the relationship between the endocan expression and age, gender, clinical stage, tumor size, lymph node metastasis, et al, to detect the expression of endocan in RKO cells stimulated by butyrate and the effect of butyrate on clonal formation in soft agar of RKO cells. Methods The 99 cases, including 27 cases of normal mucosae and 72 cases of colorectal cancer(49 cases of male, 23 cases of female; the mean age is 54, standard deviation is 15.6; 57 cases of well-differentiated, 15 cases of poor-differentiated; 27 cases of metabasis, 45 cases of non- metabasis; 43cases of A+B stage, 29 cases of C+D stage according to duke's staging), obtained from clinical operation. The mRNA expression of endocan in 27 normal colon and rectum mucosae cases and 72 cancerous cases was detected by in situ hybridization (ISH). The expression of endocan in paraffin sections of colon and rectum tissue was observated by Western blot and immunohistochemistry. The correlation between the expression of endocan and-clinical parameters was analyze via statistics software SPSS. RKO cell line was cultured and stiumulated by butyrate, then Western blot and immunocytochemistry were used to analyze the expression of endocan, and the RKO cells was treated with butyrate in soft agar to detect the clonal formation Result A high expression of endocan was detected in cytoplasm of epithelium cells. Endocan mRNA has a high expression in normal colon and rectum mucosae and a polarity distribution in the cytoplasm, the expression rate is 92.6% (25/27) ; it also has a high expression but a low...