Concentration Of Lymphocyte Immunotherapy Study Of Pregnancy Outcomes In Patients With Recurrent Miscarriage

Objectives By comparing blocking antibodies turn positive rate, the success rate of pregnancy, abortion rate and peripheral blood T lymphocyte subsets changes with different concentrations of lymphocyte immunotherapy for unexplained recurrent spontaneous abortion( URSA) patients, analyse lymphocyte concentration effects on pregnancy outcome, then explore the optimal therapeutic concentrations.Methods 1 Lymphocyte immunotherapy for URSA patients: according to whether they are willing to accept the immune treatment, we divide the patients of meeting the inclusion criteria of URSA patients into control group and test group. And the test group was divided into five groups according to the different lymphocyte immunotherapy concentration through use of healthy husband or unrelated third individuals. 2.Antibody blocking(BA) detection:the closed antibody of each group before treatment and after 1 courses of immunotherapy was detected by flow cytometry(cytometry flow, FCM) detection.3.T lymphocyte subsets changes: the changes of T lymphocyte subsets in peripheral blood were analyzed by flow cytometry(FCM) before and after 2 weeks of treatment in each group.4. pregnancy outcome: the pregnancy rate and the rate of abortion ratio were calculated 12 weeks of pregnant patients in the control group and treatment group.Results 1 After the end of the 1 course of treatment,The comparison of the positive rate of blocking antibodies in the five groups(445.6%,67.3%,86%,86.2%,87.5%),there are differences( P < 0.05).With the increase of the concentration of lymphocytes in the immune therapy, the increasing of the blocking antibody turns positive.There is a doseresponse relationship between Lymphocyte concentration and blocking antibody turns rate.When the concentration of 20 * 106/ml, the positive rate of blocking antibody turned to parallel.Comparison between 10 * 106/ml and 20 * 106/ml groups,there are differences(P<0.005).2 The pregnancy success rate of the blocking antibody positive group was86.7%,The pregnancy success rate of the blocking antibody negative group was28.9%.Pregnancy success rate in the two groups,there are differences(P<0.05).3 CD3,CD4, CD8, /CD8 CD4 before treatment,Comparison among groups,there are not differences(P>0.05),There was no significant change in T CD3 lymphocytes after 1courses of treatment,the proportion of CD4+ T lymphocytes decreased,The proportion of CD8+T lymphocytes increased,CD8+/CD4+ ratio decreased accordingly,group 1 * 106/ml and group 10 * 106/ml,20 * 106/ml,30 * 106/ml,40 * 106/ml group of four pairwise of two two comparison,there are differences(P<0.005).group 10 * 106/ml and 20 * 106/ml,30* 106/ml,40 * 106/ml group of three pairwise of two two comparison,there are differences(P<0.005),20 * 106/ml,30 * 106/ml,40 * 106/ml group among the three groups of two two comparison,no differences(P>0.005).4 Comparison of pregnancy success rate in each group( 24.2%,49.0%,69.5%,87.5%,87.5%,87.6%),there are differences(P<0.05),and the success rate of pregnancy was gradually increased with the increase of the concentration of lymphocytes in immune therapy.There is a doseresponse relationship between Lymphocyte concentration and success rate of pregnancy,But when the concentration reached 20 * 106/ml, the success rate of pregnancy tended to be parallel.Comparison between 10 * 106/ml and 20 * 106/ml groups,there aredifferences(P<0.0045).Only 2 of the patients who received the treatment had mild skin irritation and allergic reactions,no serious adverse reactions were found.Conclusions blocking antibodies turn positive rate and pregnancy success rate increases with the increase of the concentration when Lymphocyte immune therapeutic concentrations less than 20*106/ml, and changes of T lymphocyte subsets is more conducive to the pregnancy; when the concentration reached 20*106/ml, the therapeutic effect will not be significantly affected. Confirmed to ensure that immunotherapy effectiveness, the lymphocyte concentration at least to reach 20*106/ml, Low concentration has certain Tocolytic effect, but the effect is not better than high concentration.