| Objective: To investigate the effects of anti-CTLA4 antibody on Th1/Th2shift in unexplained early recurrent spontaneous abortion.Methods: The levels of IL-2,IFN-γ and IL-4 were assessed by ELISA in thesupernatant of cultured maternal peripheral blood mononuclear cells(PBMC)from 30 patients of UERSA. Cytokine synthesis was induced by activationwith antigens from a choriocarcinoma cell line of JEG-3 , with anti-CTLA4antibody or IgG.Results: We detected significantly greater levels of the Thl cytokines IL-2,IFN-γ (p<0.05)and lower levels of the Th2 cytokines IL-4 (p<0.01)inanti-CTLA4 antibody group ,compared with control group of the sameconcentration. Between the trial groups of different concentration , there is nosignificance(p>0.05).Conclusion: Anti-CTLA4 antibody can up-regulate Th1-type cytokines anddown-regulate Th2-type cytokines, which indicates that in URSAanti-CTLA4 antibody could swith T cell responses to Thl, and is harmful toURSA, while CTLA4Ig is perhaps helpful for its treatment.
|
PDF Full Text Request