Effects Of Erzhi Tiangui Granules On Kidney Yin Deficiency Type Two Elderly IVF-ET Patients With Follicular RBP-4 And Annexin A5 In Granulosa Cells

Objective: In the process of in vitro fertilization and embryo transfer(IVF-ET) observed Bushen Erzhi Tiangui Granule on senile kidney yin deficiency type infertility patients quality of oocytes, and detection of follicular fluid retinol binding protein 4(RBP-4) and ovarian granulosa cell membrane associated apolipoprotein A5(AnnexinA5) expression, from the point of view of apoptosis of kidney tonifying herbs improve kidney qi and yin deficiency type senile sterile patients oocyte quality possible mechanism.Results:(1) Compared with the control group, the experimental group, the kidney yin deficiency syndrome two have been significantly improved, the difference was statistically significant(P < 0.05);(2) patients in the experimental group of Gn dosage and number of days less than the control group, there was significant difference between two groups(P < 0.05;(3) HCG, E2) level, the experimental group was higher than the control group, there were statistically significant differences in P < 0.05), compared to the level of individual follicular estradiol, the experimental group was higher than the control group, there was significant difference(P < 0.05);(4) compared with the control group, the experimental group of oocytes, fertilization rate, egg quality rate, high quality embryo rate were higher, the differences were statistically significant(P < 0.05);(5) on the clinical pregnancy rate, compared to the two groups had no statistical significance(P=0.186 > 0.05), but the experimental group pregnancy rate significantly increased;(6) and the experimental group Compared with the control group, the expression levels of RBP-4 and AnnexinA5 in follicular fluid were increased, and the difference was statistically significant(P < 0.05).Conclusion: 1. TCM Erzhi Tiangui granules can significantly improve elderly patients with infertility of kidney yin deficiency state and joint GN infertility treatment can significantly reduce the GN dosage, shorten the duration of medication, on improving high-quality oocytes rate, high quality embryo rate effect is significant. 2. Erzhi Tiangui granules may by up regulation of follicular fluid of RBP-4 content, inhibit apoptosis of granulosa cells AnnexinA5 to improve the maturation of oocyte, so as to improve the quality of oocyte, effectively improve the elderly kidney yin deficiency infertility patients outcome of IVF treatment.