Effect Of Thyroid Stimulating Hormone (TSH) On The Growth Of Thyroid Papillary Carcinoma In Nude Mice

Objective: Long term use of thyroid hormone in patients with papillary thyroid cancer may have a major impact on cardiovascular and skeletal adverse effects. Long term TSH suppression will make the patient’s heart rate is accelerated, frequent atrial premature contraction. While the THS is less than or equal to 0.03mU/L in postmenopausal women, osteoporosis fracture risk increased 2 times[10]. About 10% of patients with PTC had a recurrence, and only a small fraction of them died of recurrence. Thyroid stimulating hormone levels in patients with thyroid papillary carcinoma should be suppressed to the number of patients who are able to benefit from the patient has not yet been clearly studied can be explained. To investigate the effects of different concentrations of thyroid stimulating hormone on the growth of thyroid papillary carcinoma, and to provide theoretical basis for clinical application of thyroid stimulating hormone inhibition.Methods: Male BALB/c-nu/nu nude mice with 35 body weight in 7⁹ g were divided into 7 groups according to the principle of complete randomization. Take human papillary thyroid carcinoma cell line TPC-1, to 1×106 cells numeration system into 100 ul cell suspension inoculation in 30 rats subcutaneously in nude mice, and were treated with different concentrations of TSH:control group（0mU/L）、2mU/L、4mU/L、8mU/L、12mU/L、16mU/L、20mU/L.The tumor size was measured and tumor size was measured, and the content of TG in the tumor tissue was determined by Radioimmunoassay method.Result:1 The cultivation of thyroid papillary carcinoma cells The culture flask was placed at 37 ℃ and in the incubator with 5% concentration of CO₂. After a large number of cell wall stickers after 4 to 5 days. Into the incubation period of papillary thyroid carcinoma cells, the cells during pseudopod extended further. The cells were polygonal, nucleus and cell volume increases gradually. 5 to 7d, the cells into the growth period of rapid proliferation, the cells continue to grow and grow to the surrounding, and connected to each other, a long spindle shaped, almost no round and oval cells, has been attached to the bottom of the culture bottle. After 7-8d, monolayer cells were formed in the culture flask. Daily observation of cell growth, 72-96 h, the cell growth density is greater than 90%, the passage of the cell. Under the microscope, the cell suspension was prepared and the cell suspension was prepared, and the number of cells in each mouse was 1 x 10⁶.2 Growth status of nude mice with different concentrations of TSH35 male BALB/c-nu/nu nude mice were injected subcutaneously on the back of the neck of TPC-1 cells, and all grew out of the tumor in 16 days. 35 nude mice were in good growth condition in 16 days and the growth of transplanted tumor was good. Their amount of activity and the amount of drinking water were not obviously abnormal.3 Comparison of tumor size in nude mice with different concentrations of TSHAccording to the measurement data,use the formula（v= length×width×width / 2） to calculate the tumor volume（unit: mm3）.And then through statistical software SPSS13.0, analysis of variance by means of measurement data. The final result（mean + standard deviation）: the 1 group:97.00±25.25、the 2 group:209.70±96.91、the 3 group:520.81±184.86、the 4 group:1398.98±97.24、the 5 group:2196.81±162.39、the 6 group:2764.33±228.29、the 7 group:3024.00±199.87. P<0.05,There was statistical significance between the groups.Volume presents an increasing trend.Multiple comparisons between multiple samples were calculated by SNK-q test.There was no significant difference between the 1 groups and the 2 groups.Volume and TSH concentration group Pearson correlation R2=0.973,They were well correlated and positively correlated.Draw a line chart.4 Comparison of the concentration of thyroid globulin in the tumor tissues of nude mice with different concentrations of TSHAfter the protein correction, through the statistical software SPSS13.0, analysis of variance by means of measurement data, the final results（mean + standard deviation）.To detect the concentration of thyroid globulin（TG） in each group（unit: ng/mgpro）:the 1 group:1.50±0.01、the 2 group:1.50v0.02、the 3 group:1.68±0.11、the 4 group:1.91±0.10、the 5 group:2.02±0.03、the 6 group:2.15±0.05、the 7 group:2.26±0.13.Through statistical software SPSS13.0, calculate the number of samples of the F test, P < 0.05, there is statistical significance between the groups.TG showed an increasing trend.Multiple comparisons between multiple samples were calculated by SNK-q test.There was no significant difference between the 1 groups and the 2 groups.Pearson correlation between TG and TSH concentration grouping R2=0.956,They were well correlated and positively correlated.Draw a line chart.Conclusion:1 TSH can improve the success rate of the establishment of the nude mouse model of thyroid papillary carcinoma.2 TSH was promoted by subcutaneous inoculation of thyroid papillary carcinoma（TPC-1） in nude mice. With the increase of the concentration of TSH, the tumor volume was increased.3 TSH has promoting effect on the secretion of thyroid globulin（TG） in the subcutaneous inoculation of thyroid papillary carcinoma cells（TPC-1） in nude mice, with the increase of TSH concentration, the concentration of TG was increased.4 The concentration of TG was positively correlated with the size of tumor.5 The effect of TSH on the growth of nude mouse in vivo of papillary carcinoma of the thyroid gland was observed for the first time,which provides a reference for the clinical treatment of TSH.