The Expression Of STAT In Allergic Contact Dermatitis Of Cosmetic

Objective:This topic through the use of imidazole alkyl urea induced cosmeti-cs allergic contact dermatitis,observed in chili and alcohol to fill the stomach in mice,imidazole alkyl urea effect on the skin,using Western blot method t o detect the mice spleen pstat3,pstat4,pstat6 expression,to explore the patho-genesis of ACD and ACD provide theoretical basis for the prevention and tre-atmentMethods:18 to 22 g,150 female kunming mice were randomly divided into 5 groups,respectively is blank group(group A)and imidazole alkyl urea grou p(group B),chili and imidazole alkyl urea imidazole group(C),alcohol and alkyl urea way(group D),(chili + alcohol)imidazole alkyl urea mixed group(group E).First A and B was given saline 10 ml/(kg · d)to fill the stomac h,C was given 10%saline 10 ml/(kg · d)to fill the stomach,D was given alcohol 56 degrees 10 ml/(kg · d)to fill the stomach,E was given 56 degrees alcohol + 10%saline mixture by 10 ml/(kg · d),observe signs of mice and score after a week.Spicy after the success of the model,A abdominal was i njected by water.And B,C,D,E mice abdominal was injected by imidazole alkyl urea.7 days later,A back with water,B,C,D,E back with 0.6%of i midazole alkyl urea,daub skin reaction after 48 h,according to the "cosmetics health standard" skin irritation experiments based on the methods and steps of operation and score.2.Take back 2 x 2 cm size in mice skin,HE staining,observed the skin pathological conditions.3.Gather the mice spleen,extracting lymphocyte after using Western blot method to detect the expression of STAT 3 and STAT4,STAT6.Results:In animal stimulated score,compared with group A,B,C,D,E,in A certain set of stimulus symptoms,higher ratings,was statistically significant(P<0.05),chili and alcohol on the body are irritating.In abnormal reaction int-ensity grading,compared with group A,B,C,D,E group score were higher,with statistical significance(P<0.05),explain in chili and alcohol under the s-timulus of imidazole alkyl urea became stronger sensitization.Mice back to are as with histopathologic grading,according to the results of group A did not ca use skin tissue pathological changes,score of 0.The rest of the four groups c ompared with group A score were higher,statistics have significant difference(P<0.05),explain in edible chili and alcohol cases,cosmetic allergic contact dermatitis can aggravate symptoms.Spleen in Western blot test results showed that B,C,D,E group compared with group A,the STAT3,STAT4 expression are increased,statistically significant differences(P<0.05),explain in cosmeti c allergic contact dermatitis,STAT3,enhanced STAT4 expression.Conclusion:1.Alcohol caused increasing imidazole alkyl urea CACD ability bet-ter than pepper.2.STAT3 and STAT4 expressed in CACD,pepper and alcohol can increase the spleen in mice The expression of STAT3 and STAT4 that may be related to promote ThO to Thl cells,Th17 cell differentiation.3.As a result,this paper argues that the onset of CACD and Thl,Th2,Th17 cells.