Synthesis And Properties Of Fluorescent Probes Based On Rhodamine And Coumarin Dyes

Spectrofluorometry plays an important role in the field of the production research, food inspection, environmental monitoring and drug analysis and life scientific reaearch. However it is limited in many fields for spectroscopical silent substances (such as most of inorganic substances) or weakly fluorescent substances. It is significant to explore more novle dyes or probes with high sensivity and selectivity to determine metal ions (especially heavy metal ions). According to the increase or decrease of fluorescence intensities when these ions are binded with a fluorescent probe. In detail, four major works have been completed in the thesis. The content of each chapter in the thesis are summarized as the following:Chapter 1:The design principle of fluorescent molecular probes are introduced. Development and application of rhodamine and coumarine flurescent probes are reviewed.Chapter 2:A novel fluorescent reagent 3',6'-bis(diethylamino)-2-(4-(diethyl lamin o)-benzylideneamino)spiro[isoindoline-1,9'-xanthen]-3-one was confirmed by infrared spectra (IR), elemental analysis, mass spectrometry (MS),1HNMR and 13CNMR. The results show that the probe can selectively combine with Cu (II) for the determination of trace Cu (?). Under the optimal conditions, there is a good linear relationship between the increasement fluorescence intensity and the concentration of Cu (?). Trace Cu (?) in lake water and fruit juice was determined with satisfication result.Chapter 3:A novel fluorescent reagent 3',6'-bis (diethylamino)-2-(4-(dimethyl amino) benzylideneamino) spiro [isoindoline-1,9'-xant hen]-3-one was confirmed by infrared spectra (IR), elemental analysis, mass spectrometry (MS),1HNMR and 13CNMR. The results show that the probe can selectively combine with Cu (?) for the determination of trace Cu (?). Under the optimal conditions, there is a good linear relationship between the increasement fluorescence intensity and the concentration of Cu (?). A new method for the determination of copper ion was provided here.Chapter 4:A novel fluorescent reagent 3',6'-bis (diethylamino)-2-(quinolin-2-y lmethyleneamino) spiro [isoindoline-1,9'-xanthen]-3-one was confirmed by infrared spectra (IR), elemental analysis, mass spectrometry (MS),1HNMR and 13CNMR. The results show that the probe can selectively combine with Al (?) for the determination of trace Al (?). Under the optimal conditions, there is a good linear relationship between the fluorescence intensity and the concentration of Al (?). A new fluorescence detection method for aluminum ion was constructed.Chapter 5:A novel fluorescent reagent 7-(diethylamino)-N'-(4-(dimmethylamino) benzylidene)-2-oxo-2H-chromene-3-carbohydrazidewas confirmed by infrared spectra (IR), mass spectrometry (MS),1HNMR and 13CNMR. The results show that the probe can selectively combine with Cu (?) for the determination of trace Cu (?). Under the optimal conditions, there is a good linear relationship between the fluorescence intensity and the concentration of Cu (?). A new fluorescence method for the detection of copper ion was constructed.