The Expression Of IL-26 In Gastric Cancer And Its Diagnostic Value

Objective:Discuss the IL-26 expression differences and gene expression differences between gastric cancer tissues and adjacent normal tissues in gastric patients and the IL-26 expression level differences in peripheral blood between gastric cancer patients group and normal people group,then explore the relationship between IL-26 and its biological characteristics.Finally, Combined with the level of expression of the same period of gastric cancer commonly serum tumor markers CEA,CA19-9,CA24-2 and CA72-4 to explore the serum IL-26 value in the diagnosis of gastric cancer.Method:1.50 cases of pathological tissue derived from surgical gastric cancer patients in our hospital were selected as study object to assess the IL-26 expression differences between the adjacent tissues and normal tissues by Image Pro Plus semi-quantitative method in immunohistochemistry.Analysis the m RNA expression of IL-26 between gastric mucosal tissue and cancer adjacent normal gastric mucosa by RT-PCR test,which was analysed by MannWhitney U test.2.176 cases of peripheral blood derived from gastric cancer patients and 176 cases of that derived form normal individuals who had taken clinical examination were collected as research object,and then detected the clinical serum IL-26 and serum tumor markers in the diagnosis of gastric cancer in the same period including:CEA,CA19-9,CA24-2 and CA72-4,the expression level had statistical significances by enzyme-linked immunosorbent assay and such differences Had statistically significance.3. Acquiring a large clinical data to assess the correlation of serum IL-26 and the biological characteristics of gastric cancer patients.4. The sensitivity and specificity of IL-26 were count out by the ROC curve Diagnostic tests, and compared with the conventional diagnosis of gastric cancer serum tumor markers CEA,CA19-9,CA24-2 and CA72-4 diagnosis indicators for comparative analysis,and finally assessed diagnosis value in gastric cancer detection when United wih CEA,CA19-9,CA24-2 and CA72-4.Results:1.The results derived from the immunohistochemical experiment in gastric cancer tissues and adjacent normal tissues showed that positive expression ratio in gastric carcinoma presents was 100%(50/50) of IL-26, and positive expression ratio in the adjacent normal tissue was 70%(35/50) of IL-26.The average OD value was calculate by the Image-Proplus 6.0 statistical software.Gastric cancer average OD value was 0.25 ± 0.11, and adjacent normal tissue average OD value was 0.09 ± 0.05. compared the differences between the two groups by t test, then grastric cancer was higher than adjacent and the difference was statistically significant(P<0.001). The m RNA expression of IL-26 in gastric mucosa tissue of gastric cancer patients was significantly higher than that in normal gastric mucosa tissues by non parametric Mann Whitney U test(P<0.05).2.The outcome showed that gastric cancer serum IL-26 median is 279.89 pg/ ml [(241.21 ~ 301.38) pg / ml], normal IL-26 median is 216.10 pg /ml[(172.13~ 264.09) pg / ml]. Compared with the normal group, the IL-26 gastric cancer group was significantly increased(P<0.05),which is observed by the enzyme-linked immunosorbent assay.3.The results of biological characteristics of gastric cancer patients with clinical and pathological data,the age of 60 years or more,or equal to no significant difference in the expression levels of serum IL-26 in patients withage less than 60 years old 60 years in(P> 0.05),male and female patients with gastric cancer in patients with gastric cancer had no significant difference(P>0.05) in the serum levels of IL-26 expression, the expression of serum IL-26 in gastric cardia region and which parts of the region of non-cardia gastric cancer no significant difference(P>0.05)on the level of cancer patients with gastric cancer and greater than 5cm or less there had difference(P<0.05) in the expression levels of serum IL-26, and greater than or less than 5cm of gastric cancer high levels of IL-26 expression, invasion depth in the T1/T2 and not in patients with gastric cancer T3/T4 in serum IL-26 on the expression level of significant difference(P>0.05),in patients with intestinal type gastric cancer Lauren and the absence of IL-26 expression levels in serum of patients with diffuse gastric cancer on the significant difference(P>0.05),in patients with Helicobacter pylori positive and negative there is no significant difference(P>0.05) on IL-26 expression levels lymph node metastasis and lymph node-negative IL-26 expression levels were not significantly different(P>0.05),while in the early clinical stage and clinical stage advanced subjects on IL-26 expression level differences(P<0.05) and in patients with advanced gastric cancer serum IL-26 expression levels higher than clinical staging of early gastric cancer expression level, summarizes the serum levels of gastric tumor size and IL-26,and clinical stage(P<0.05), their age, gender,tumor location,depth of invasion,Lauren classification,lymph node metastasis, Helicobacter pylori infection(P>0.05) showed no significant correlation.4.The results of gastric cancer and normal group serum IL-26 by the ROC curve were as follows: the AUC was 0.93,according to the fact that the area under the principle of statistical curve(AUC)>0.9 was high diagnostic accuracy,corresponding to the maximum by the Youden index was 252.76 pg /ml as the cut off value, obtained 79.2% diagnostic sensitivity, diagnostic specificity of 87.4%(P=0.007).The same gastric cancer patients serum CEA,CA199, CA242 and CA724 from AUC are 0.85,0.68,0.82 and 0.71,diagnosticsensitivity were 56.3%, 38.6%, 41.3% and 62.4%,the diagnostic specificity were 96.1%,93.9%,95.9% and 85.2%.When IL-26 combined with common gastric tumor marker CEA, CA724, CA199 and CA242,diagnostic sensitivity of that was 89.2%,diagnostic specificity of that was 97.6%, the overall the sensitivity and specificity were improved than any single examination.Conclusions:1.Assessed resected pathological tissues of cancer patients in surgical treatment,then confirmed that the IL-26 and its gene in gastric cancer portion pathological tissue compared to normal tissue had significantly overexpressed,which may be associated with the change of gastric cancer microenvironment,in the role of chemokines,IL-26 primarily secreted by Th17 is increase in local tissue,which is caused by Th17 cell recruitment.2.Serum IL-26 in the gastric cancer patients with respect to the same period in the normal population group showed high expression and the difference had statistically significant,biology and clinical message from gastric cancer patients were collection,that includes tumor size, clinical stage,age, gender,tumor location, depth of invasion, Lauren classification, lymph node metastasis,Helicobacter pylori infection, analysis results show that their age, sex, tumor location,Lauren classification,lymph node metastasis,Helicobacter pylori infection have no significant correlation,but the serum IL-26 levels in gastric cancer patients have relationship with tumor size and clinical stage,also that difference has statistically significant.the phenomenon which can taken IL-26 as serum markers provide basement.3.Judging diagnosis value of serum IL-26 expression levels,gastric cancer showed higher sensitivity than serum tumor markers CEA,CA19-9, CA24-2and CA72-4 in the same period on the extensive use of the clinical routine,and diagnostic specificity was maintained in a relatively good level,United serum IL-26 with gastric conventional tumor markers CEA, CA19-9, CA24-2and CA72-4 can improve diagnostic performance. joint detection had been improved than single detection, but still widely used in clinical should get a comprehensive analysis of the expansion of sample number and the multi-regional and multi-center to proved its reliability and practicality.