The Role And Mechanistic Investigation Of Mixed Powder Ginseng And Auricularia On Hypolipidemic Activity

Purpose:Discussing the fabrication technology of Ginseng powder and complex enzymatic preparation technology of Auricularia Polysaccharide. Study the hypolipidemic function of Ginseng and Auricularia Powder and its mechanism.Methods:1. Studies on preparation process of Ginseng and Auricularia Powder(1) Using micronized technology to obtain micronization ginseng powder, and investigating the influence of wind, grinding time and feedstock amount on Ginseng micronization, taking the average particle size, and bulk density, angleofrepose, powder production and output ratio as the standard.(2) Using complex enzyme method to extract Auricularia Polysaccharide, and investigating the effects of solid to liquid ratio, proportion of enzymes, reaction time, temperature, extraction time, extraction times on the extraction of Auricularia Polysaccharide, taking polysaccharide extraction rate as the index.2. Research on hypolipidemic effect of Ginseng and Auricularia Powder(1) Influence of Ginseng and Auricularia powder on content of TC, TG, HDL-C, LDL-C in hyperlipidemia mice serum.(2) Influence of Ginseng and Auricularia powder on content of TC, TG, HDL-C, LDL-C in hyperlipidemia rat serum.3. Research on hypolipidemic mechanism of Ginseng and Auricularia Powder(1) Adsorption experiments in vitro of Ginseng and Auricularia Powder1) Measure the water holding capacity, expansion force and oil absorption capacity of Ginseng and Auricularia Powder;2) Measure the adsorption capacity of cholesterol in egg yolk;3) Measure the adsorption capacity of nitrite ion.(2) Studies on the effects of Ginseng and Auricularia Powder in vivo adsorption experiment1) Measure the content of cholesterol in liver and feces of rats with hyperlipidemia;2) Measure the content of bile acid in liver and feces of rats with hyperlipidemia;(3) Measure the oxidation resistance of Ginseng and Auricularia Powder1) The effect of Ginseng and Auricularia Powder on SOD in serum of rats with hyperlipidemia;2) The effect of Ginseng and Auricularia Powder on MDA in serum of rats with hyperlipidemia;3) The effect of Ginseng and Auricularia Powder on GSH-PX in serum of rats with hyperlipidemia;(4) The effect of Ginseng and Auricularia Powder on mice small intestinal movement.Results:1. The research result of Ginseng and Auricularia Powder preparation technology(1) The best preparation technology of ginseng powder: Single feedstock amount of 500 g, the length of pant’s leg of 20 cm, crusher time of 40 min.(2) The best preparation technology of Auricularia polysaccharides: the ratio between material and water of 1:40, the addition ratio of complex phosphoesterasum of 2%, extraction temperature of 100 ℃, extraction time of 2h, and the highest yield. 2. Research on the hypolipidemic function of Ginseng and Auricularia Powder(1) Ginseng and Auricularia Powder can reduce the content of TC, TG, and LDL-C in serum of hyperlipidemia mice, increased the content of HDL-C in serum of hyperlipidemia rats.(2) Ginseng and Auricularia Powder can reduce the content of TC, TG, and LDL-C in serum of hyperlipidemia rats, increased the content of HDL-C in serum of hyperlipidemia mice.3. Research on the hypolipidemic mechanism of Ginseng and Auricularia Powder(1) The research result of Ginseng and Auricularia Powder in vitro adsorption experiment1) The water holding capacity of Ginseng and Auricularia Powder is about 1/4 of Auricularia; the expansion volume of Ginseng and Auricularia Polysaccharide Powder after water absorption is much smaller than Auricularia; the oil-absorbing ability of Ginseng and Auricularia Polysaccharide Powder is twice of Auricularia;2) Under the same conditions, the adsorption capacity of cholesterol in egg yolk of Ginseng and Auricularia Powder is three times of Auricularia, and then reduce the cholesterol absorption of human body from food;3) Under the same conditions, the adsorption capacity of nitrite of Ginseng and Auricularia Powder is three times of Auricularia, and then reduce the injury of nitrite on the human body.(2) Studies of absorption experiment in vivo of Ginseng and Auricularia Powder1) Ginseng and Auricularia Powder can reduce the content of TC and TG in liver and increase the content in feces.(3) Results of antioxidant capacity tests of Ginseng and Auricularia Powder1) Ginseng and Auricularia Powder can increase the level of SOD in the serum of hyperlipidemia rats;2) Ginseng and Auriculariae Powder can reduce the level of MDA in the serum of hyperlipidemia rats;3) Ginseng and Auricularia Powder can increase the level of GSH-PX in the serum of hyperlipidemia rats;(4) The effect of Ginseng and Auricularia Powder on mice intestinal movement1) Ginseng and Auricularia Powder can promote the intestinal movement in mice with constipation. Conclusion:1. Optimum preparation technology of Ginseng and Auricularia Powder. Optimum preparation items of Ginseng powder are that, the feedstock amount is 500 g, the length of air bags is 20 cm and the grinding time is 40 min; Optimum preparation items for extraction of Auricularia Polysaccharide are that, the ratio of feedstock and water is 1:40, the ratio of enzyme is 2%, the extraction temperature is 100 ℃ and the extraction time is 2 h. Then mixing the ginseng powder and Auricularia polysaccharide with the ratio of 1:2.2. Ginseng and Auricularia Powder improves hyperlipidemia in mice and regulates the changes of serum lipid in rats.3. Hypolipidemic mechanism of Ginseng and Auricularia Powder may relate to the reducing of cholesterol absorption in intestinal, increasing of the cholesterol exchange in the body and reducing of cholesterol synthesis.