Extraction And Antibacterial Ability Of Hosta Ventricosa Roots Of The Active Ingredient

Hosta ventricosa Hosta Liliaceae perennial herb, widely distributed in Northeast China, is a common ornamental plants. Studies have shown that it can also be applied treatment carbuncles, burns, sore throat and other diseases, Is also a highly valuable medicinal plants. In this experiment, Hosta ventricosa root for the study, Hosta ventricosa root extract active ingredients, determine the optimum conditions; By column chromatography on Hosta ventricosa roots triterpenoids and polysaccharides were isolated and purified; Verify that the main component of Hosta ventricosa roots flavonoids and triterpenoids by high performance liquid chromatography; Finally, Extraction of active ingredients antibacterial test, Detection of inhibitory effect of each active ingredient, Determine the minimum inhibitory concentration. The results are as follows:1. The application of ultrasonic extraction method to determine the optimum extraction conditions Hosta ventricosa roots of active ingredients. The results showed that: Hosta ventricosa roots flavonoids optimum extraction conditions: Ethanol concentration of 80%, temperature of 80℃, time of 60 min, solid-liquid ratio 1:15; extraction rate of 25.48%. The optimum extraction conditions Hosta ventricosa roots triterpenoids of: Ethanol concentration of 70%, a temperature of 70℃, time was 100 min, solid-liquid ratio of 1:10; extraction rate of 43.21%. Hosta ventricosa roots alkaloids optimum extraction conditions: Ethanol concentration of 70%, the temperature is 80℃, time of 60 min, solid-liquid ratio 1:15; extraction rate of 7.3%. Hosta ventricosa roots polysaccharide optimum extraction conditions: Temperature is 80℃, time was 100 min, solid-liquid ratio 1:15; extraction rate of 77.08%.2. Application column chromatography on Hosta ventricosa roots triterpenoids and polysaccharides were isolated and purified. The results showed that: Triterpenoids isolated two components: TPD-A and TPD-B, the yield was 21.4% and 48.3%, respectively. Only two components are eluted relatively single symmetrical peak, description of these two components has a certain similarity. Polysaccharides also isolated two components: Y1 and Y2, the yield was 62.5% and 32.2%, respectively. Eluent is collected by high temperature solvent evaporated to dryness to give a crystal cube, this is a preliminary identification of a mixture of sodium chloride and polysaccharides.3. The main component of high performance liquid chromatography to verify Hosta ventricosa roots flavonoids and triterpenoids in. The results showed that: Hosta ventricosa roots main ingredient is flavonoids rutin, The main component of triterpenoids are oleanolic acid.4. Hosta ventricosa roots extract active ingredients in antibacterial test. The results showed that: ① Hosta ventricosa roots have antibacterial effects. ②Antibacterial ingredients are flavonoids, triterpenoids and polysaccharide. ③Flavonoids, triterpenoids and polysaccharides the four tested strains have antibacterial effects. ④Antibacterial activity of flavonoids in E.coli>Bacillus subtilis>Salmonella>Staphylococcus aureus; Wherein, Escherichia coli, Bacillus subtilis minimum inhibitory concentrations of 25mg/ml, 12.5mg/ml, Staphylococcus aureus, minimum inhibitory concentration of Salmonella are 100mg/ml.⑤ Antibacterial activity of triterpenoids as Bacillus subtilis> Salmonella>E.>Staphylococcus aureus; Wherein, Escherichia coli, Bacillus subtilis, Staphylococcus aureus and Salmonella minimum inhibitory concentration was 12.5mg/ml, 25mg/ml, 100mg/ml, 50mg/ml. ⑥Polysaccharide antibacterial activity of Bacillus subtilis>E.>Staphylococcus aureus>Salmonella; Wherein the minimum inhibitory concentration of Escherichia coli, Staphylococcus aureus were 12.5mg/ml, 50mg/ml, Bacillus subtilis, Salmonella are the minimum inhibitory concentration of 25mg/ml. ⑦ Various active ingredients for E. coli, Bacillus subtilis inhibitory effect was significantly higher than that of the role of Staphylococcus aureus, Salmonella.