Studies On Extraction, Separation And Purification Techniques Of Active Ingredients In Eucommia Ulmoides Oliv

The Eucommia belongs to the Eucommia ulmoides Oliv, and was originally recorded in《Sheng Nong's herbal classic》. Eucommia is a traditional Chinese cash crop and has prolific chemical substances, such as phenylpropanoids, iridoids, lignans etc; and has various medical efficacy, such as protecting liver, anti-bacteria and detoxification, enhancing immunity, reducing blood fat and blood pressure etc. The paper studies on the extraction, separation and purification techniques of the active ingredients in Eucommia ulmoides oliv and to acquire the best routing for the deeply process of Eucommia and deeply researches how to establish the best producing process for Eucommia1) Study on the extraction techniques in Eucommia bark. By comparing the three kinds of extraction method (the soxhlet extraction, the direct extraction and the ultrasonic extraction), the experiment result indicates that the ultrasonic extraction is the best one. It is a quick and efficient method and the best production technique has been established.2) A breakthrough method of reversed-phase high performance liquid chromatography has been established for the simultaneous detection of geniposidic acid, geniposide, chlorogenic acid and pinoresinol diglucoside in Eucommia ulmoides. Separation was achieved using a Kromasil C18 column (4.6 mm×200 mm,5μm), under the gradient elution with the mobile phase of acetonitrile and 0.1%(v/v) phosphoric acid/water, linear gradient:0-7min,7%B, 7-35min,14%B,35-45min,15%B, flow rate was 1.0 mL/min, detected at 235 nm and under 30℃. The linear ranges of 4 components were 0.1025-0.5125μg/mL (R2=0.9999),0.0925 0.4625μg/mL (R2=0.9999),0.120-0.600μg/mL (R2=0.9996),0.090-0.450μg/mL (R2=0.9998) respectively. The average recovery rate (n=5) is 96.06%,96.22%,96.12%,96.08% and with RSD of 1.39%,1.82%,1.53% and 1.59% respectively. The method is simple, rapid and highly sensitive, suitable for the quality control of Eucommia ulmoides and its health products.3) The separation and purification techniques of pinoresinol diglucoside and chlorogenic acid in eucommia ulomide oliver, In the 9 kinds of materials to choose a suitable macroporous, it is NAK-1 and absorption resins to enrich the active compound from Eucommia. adding original extraction (40mL, pH3-4,5-6) to XDA-I colum, stewing 6h, the washed with pure water, flow rate of adsorption was control at 1 mL/min, chlorogenic acid use 40% alcohol, pinoresinol diglucoside use 80% alcohol to elution (volume4BV,7BV) was concentrated and dried.