The Extraction Process Of Tanggut Rhubarb And The Chemical Separation And Antibacterial Activity Of Water Extracting Parts

Rheum tanguticm Maxim Ex Balf is Polygonaceae rhubarb Rheum perennial herbaceous plants,mainly distributed in Qinghai,Gansu,Sichuan northern and other places in China,is one of the major traditional Chinese medicine of traditional Chinese commonly used.Rhubarb not only have spilled hot bowel,cooling blood detoxification,but also have diarrhoea,anti-inflammatory,lower blood pressure,antibacterial,antivirus,antitumor,fall fat,reduce weight,improve insulin resistance,and other functions.Using response surface method to optimize the extraction conditions of Rheum tanguticm Maxim Ex Balf anthraquinone,get the best extraction technology conditions of rhubarb anthraquinone,The optimum extraction conditions are 70 % ethanol concentration,extraction time79 min,extraction temperature79 ℃,material liquid 1:12.Rhubarb free anthraquinone extraction yield of 0.09%.The column chromatography isolated from parts of Rheum tanguticm Maxim Ex Balf the water area including 9 kinds of compounds,through hydrogen nuclear magnetic spectrum analysis technology,respectively : rhein-8-O-β-D-glucopyranside(Ⅰ).Emodin-8-O-β-D glycosidase(Ⅱ),aloe emodin-1-O-β-D pyran glycosidase(Ⅲ),aloe emodin(Ⅳ),rhein(Ⅴ),catechins(Ⅵ),emodin(Ⅶ),physcion(Ⅷ),chrysophanol(Ⅸ).Compound Ⅰ10 kinds of pathogenic bacteria of cholecystitis bacteriostatic effect compared with negative control group significantly(P < 0.01),compared with positive control group compound Ⅰon staphylococcus aureus and excrement enterobacter bacteriostatic effect was not significant(P > 0.05)were extremely significant bacteriostatic effect of other bacteria,especially for sewer enterobacter sewer subspecies,strong bacteriostatic action of subspecies of pneumonia klebsiella pneumonia bacteriostasis weaker.Compound Ⅱcholecystitis pathogenic bacteria in 10 bacteriostatic effect compared with negative control group significantly(P < 0.01),compared with positive control group bacteriostatic effect is also very significant(P < 0.01),especially for staphylococcus aureus,strong bacteriostatic action of pneumonia klebsiella pneumonia subspecies bacteriostasis weaker.Compound Ⅲpathogenic bacteria of 10 kinds of cholecystitis bacteriostatic effect compared with negative control group significantly(P < 0.01),compared with positive control group,the sewer enterobacter sewer subspecies bacteriostatic effect significantly(P < 0.05)on other bacteria antibacterial effect is very significant,especially for staphylococcus aureus,strong bacteriostatic action of subspecies of pneumonia klebsiella pneumonia bacteriostasis weaker.Compound pathogenic bacteria of 10 kinds of cholecystitis Ⅴbacteriostatic effect compared with negative control group significantly(P < 0.01),compared with positive control group bacteriostatic effect is also very significant(P < 0.01),especially for staphylococcus aureus,strong bacteriostatic action of the excrement enterococcus bacteriostatic action.For staphylococcus aureus,compound Ⅴ its bacteriostasis stronger;For streptococcus faecalis,compound Ⅰits bacteriostasis stronger;For e.coli,compound Ⅲfor its strong bacteriostatic action;For pneumonia klebsiella pneumonia subspecies,compound Ⅴfor its strong bacteriostatic action;For gas enterbacteriaceae compound Ⅱits bacteriostasis stronger;For drain enterbacteriaceae sewer subspecies,compound Ⅴfor its strong bacteriostatic action;For proteus,compound Ⅴ for its strong bacteriostatic action;For producing acid klebsiella,compound Ⅰfor its strong bacteriostatic action;For pseudomonas aeruginosa,compound Ⅴon its bacteriostasis is stronger,for the excrement enterococcus,compound for its strong bacteⅢ riostatic action.