The Research About Gene Polymorphism Of α-synuclein In Parkinson’s Disease

Objective According to the genetype analysis of the α-synuclein genetic variation A30 P 、 A53 T 、 rs3831458 to evaluate the risk of PD and the severity of PD patients’ condition. Method(1) The genetic variation A30P、A53T、rs3831458 of α-synuclein gene were carried out by PCR and DNA sequence analysis, then grouping with diferent H-Y Staging Scales,was developed to amplify short tandem repeat(STR) in combination with Chinese Parkinson′s disease patients and normal controls;(2) Using SPSS 19.0 and chi squared test to analyse the determinant factor about the risk of PD and the severity of PD patients’ condition. Results(1) A30 P was statistical analysis in 264 PD patients and146 normal controls. It could conclude: Dominant allele A30P-C(P<0.05,OR=1.863) was associated with PD and made the risk increase by 1.863-fold; Dominant allele A30P-G(p<0.05,OR=0.537) could made the risk decrease by 0.537-fold;Homo-zygote A30P-C/C(P<0.05,OR=2.308) was associated with PD and made the risk increase by 2.308-fold, it also had signification in H-Y ≥3(P<0.05,OR=1.900) 、 H-Y≤2.5(P<0.05,OR=3.667);Homoz- ygote A30P-G/G(P<0.05,OR=0.556) could made the risk decrease by 0.556- fold,it also had signification in H-Y≥3(P<0.05,OR=0.546). Furthermore it concluded that A30 P was not associated with the severity of PD patients’ condition by the statistical analysis between H-Y≥3 and H-Y≤2.5 PD patients.(2) A53 T was statistical analysis in 224 PD patients and 154 normal controls. It could conclude: Dominant allele A53T-A(P<0.05,OR=1.889)was associated with PD and made the risk increase by 1.889-fold;Dominant allele A53T-G(P<0.05,OR= 0.529)could made the risk decrease by 0.529-fold;Homo-zygote A53T-A/A(P<0.05,OR=3.130) was associated with PD and made the risk increase by 3.130- fold, it also had signification in H-Y≥3(P<0.05,OR=2.465) 、H-Y≤2.5(P<0.05,OR=9.286); Homoz- ygote A53T-G/G(P<0.05,OR=0.553) could made the risk decrease by 0.553-fold, it also had signification in H-Y≥3(P<0.05,OR=0.507).Furthermore it concluded that A53 T was not associated with the severity of PD patients’ condition by the statistical analysis between H-Y≥3 and H-Y≤2.5 PD patients.(3)rs3831458 was statistical analysis in 95 PD patients and 95 normal controls. It couldconclude:-/CT(P<0.05,OR=1.911) was associated with PD and made the risk increase by1.911-fold; it also has signification in H-Y≥3(P<0.05,OR=3.125). AG/CT(P<0.05,OR=0.523) could made the risk decrease by 0.523-fold, it also has signification in H-Y≥3(P<0.05,OR=0.320). Furthermore it concluded that rs3831458(P<0.05,OR=2.456) was associated with the severity of PD patients’ condition by the statistical analysis between H-Y≥3 and H-Y≤2.5 PD patients, it made the risk increase by 2.456-fold. Conclusions(1) Dominant allele A30P-C 、Homozygote A30P-C/C were risk to PD, maybe produce PD; Dominant allele A30P-G 、 Homo-zygoteA30 P G/G may be protective factors attenuated the incidence of the disease rate.(2) Dominant allele A53T-A 、Homozygote A53T-A/A were risk to PD, maybe produce PD; Dominant allele A53T-G、Homo-zygote A53 T G/G may be protective factors attenuated the incidence of the disease rate.(3)rs3831458-/CT was risk to PD, maybe produce PD and accelerate the progress of PD patients’ condition. AG/CT may be a protective factor attenuated the incidence of the disease rate.