Study On Extraction And Purification Process Of Flavonoids From Pleurotus Eryngii And Its Antioxidant Activities

Flavonoids is a kind of secondary metabolites, which widelyexists in plants. Flavonoids have many bio-activity, such asantibacterial，anticancer，antioxidation and so on. At present, thestudy of flavonoids has gradually becoming the hot of secondarymetabolites. In this study, the technologies of extraction andpurification the flavonoids compounds, as well as antioxidativeresearch, and provide the technical base to comprehensiveutilization and resource development of Pleurotus eryngii. The mainresults are shown as follows:(1) The method of NaNO2-Al(NO3)3colorimetric was used tomensutated of the content of FLP. The precision, reproducibility andstability test are satisfactory, the RSD were1.8%(n=5),3.93%(n=5),3.78%(n=5). The average recoveries were94.7%, RSD was3.55%(n=5).(2)The extraction condition of flavonoids from Pleurotus eryngiiby ultrasoung were studied in this paper. Based on the results ofsingle-factors test, the Response Surface Methodology (RSM) wasapplied to optimize the extraction of flavonoids from Pleurotuseryngii flavonoids from Pleurotus eryngii of optimum extraction conditions were as follows,80%of the ethanol concentration,liquid-solid ration of1:40, ultrasonic temperature of80℃, ultrasonictime100min. Under these conditions, the flavonoids extraction was2.92%.(3)To study the adsorptive property from D101、AB-8、X-5、D4020of macroreticular resin to flavones by the index of adsorption rate,desorption rate and kinetic curve. The results showed that the typeof X-5macroreticular resin had the superior purification effect,which was the optimal type for purifying.(4)To invesitage the concentration of sample/liquid, dosage ofsample/liquid and concentration and quantity of eluent to affectflavones of Pleurotus eryngii in the experiment of the type of x-5macroreticular resin purified flavones, the optimum conditions werethe concentration of extracting solution was0.6mg/mL, the tatio ofsample to resinous was15:1,70%ethanol solution as the eluent andthe dosage was5BV.(5)The content of flavonoid in the different polarity parts fromPleurotus eryngii was determined. The results show that thecontent of flavonoid in chloroform fraction is51.73%which is thehighest among the4different polarity parts. The content offlavonoid in ethyl acetate fraction is46.06%which is higher thanchloroform fraction and water fraction. And the content of flavonoidin water saturation normalbutyl alcohol fraction and petroleumether is36.34%and20.34%. By measuring the reducing power ofthe extract, clear indicators of free radical scavenging(O2-·、HO-·、DPPH·、 ABTS·+), anti-lipid oxidation capacity, to study theantioxidant activity in vitro simulation system. The results show that, different polar fractions have some antioxidant activity, andincreases with increasing concentration.