| ObjectiveObservation of soothing liver and tonifying the kidney to improve elderly IVF patients oocyte quality and follicular fluid SCF(stem cell factor level relations from improve SCF(stem cell factor) and explore the angle to the horizontal in soothing liver and tonifying the kidney to improve the mechanism of the embryo implantation rate.Method1. In accordance with the order of patient visits in a random number table will receive 60 cases of Stagnation Syndrome is a kidney IVF-ET progesterone elderly patients were divided into treatment group, 30 patients in the control group of 30 patients. Two groups were pretreated three menstrual cycles. Kidney Liver Depression and infertility observed in elderly patients symptom scores change; gonadotropin usage(Gn) observed in patients, injection of HCG day serum E2, LH, P level; statistical number of patients eligible for eggs, egg excellent rate, fertilization rate and quality embryo, implantation rate. 2. Patients with follicular fluid collected oocyte day, the collected follicular fluid level detection SCF enzyme-linked immunosorbent assay(ELlSA).Results1. After treatment, kidney liver depression symptoms improved; the treatment group and the control group before and after treatment of kidney liver depression syndrome integral difference, the difference was statistically significant(P = 0.000 <0.05). 2. HCG day treatment group E2 / oocytes was significantly higher, the difference was statistically significant(P = 0.001 <0.05), LH was no significant difference(P = 0.330> 0.50, but P was significantly lower than the control group, the difference There was statistically significant(P = 0.039 <0.05)). 3. The control group of high-quality eggs in the treatment group, good quality embryo, implantation rates lower, the difference was significant(P <0.05). The pregnant group compared with non-pregnant patients: further stagnation of liver and kidney were divided into pregnant and non-pregnant group, the group of high-quality egg pregnancy rate, high-quality embryos were higher than the non-pregnant group, the difference was significant(P <0.01). 5. The treatment group compared to the control group of low follicular fluid SCF level, the difference was significant(P <0.05). 6. HCG injection day patient serum E2 levels and follicular fluid levels of SCF correlation analysis showed that SCF and E2 levels were positively correlated(p <0. 0l). 7. The number of days the amount and use of the experimental group was significantly lower than the control group Gn, quality of eggs, fertilization rate, good quality embryo, implantation rate was significantly higher differences were statistically significant(P <0.05), oocyte number of differences with the clinical pregnancy rate was not statistically significant(P> 0.05), but the test group and the control group had an increasing trend.Conclusion1. In IVF-ET treatment cycle, the application of Bushen Shugan medicine can significantly improve the kidney deficiency and liver depression in patients with kidney deficiency symptoms and ovarian reproductive endocrine function. 2. Experimental group quality egg rate, fertilization rate and good quality embryo rate was significantly higher than that of control group, non pregnant group follicular fluid SCF level lower pregnancy group, suggesting that ovum fertilization rate and embryo quality may be affected by dry cytokine levels in follicular fluid, high level of stem cell factor helps to egg fertilization and the formation of high-quality embryos. 3. Compared with the control group, the treatment group follicular fluid SCF level higher, suggesting that soothing liver and tonifying the kidney can improve follicular fluid SCF level, and improve the elderly IVF patients with follicular development quality, and increase embryo implantation rate. |
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