The Pharmaceutical Study Of Anti-alcoholic Liver Injury Periplaneta Americana Tablet

Alcoholic liver disease is the liver damage lesions which caused by long term, a large number of drinks containing all kinds of drinks ethanol, the incidence of the disease is presenting the trend of rising year by year around the world. It has become one of the clinical common disease, frequently-occurring disease, seriously affected the quality of people’s lives, the prevention is imminent.This topic, according to the etiology and pathogenesis of alcoholic liver damage, combined with syndrome differentiation of traditional Chinese medicine, we choose Periplaneta Americana as a model drug, study the effect of efficacy in treatment of alcoholic live disease. Then we determine the extraction method. On the basis of the research, we study and manufacture periplaneta Americana tablets, in order to provide clinical application.First of all, we research the preliminary study of Periplaneta Americana on the pharmacodynamics to alcoholic liver disease. Alcoholic liver damage model is established, the serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma GGT (gamma glutamyl transpeptidase) activity (GGT) in mice serum were measured, and liver coefficient was calculated. At the end of the treatment, hepatic tissues were conduct to the histopathological examination. To explore the effect of Periplaneta Americana herbs and extractive against Hepatic Injury Induced by Alcohol in mice, for the industrial production of Periplaneta Americana provide a theoretical. From the pharmacodynamics, alcohol extractive and water extractive and alcohol water area have a certain therapeutic effect, acohol water extractive works best. The fat-soluble components have some certain side effects to alcoholic liver injury.With content of protein, carbohydrate and extract rate as the evaluation index, we use single factor to optimize the ethanol extraction process, add10times the amount of85%ethanol, extracting3times, each time1h. Then we inspect the enrichment and degreasing process, determine at60℃to concentrate1:1(the original medicinal herbs:extract), cold storage method to remove fat soluble ingredient. We take the residue to optimize water extraction technology. We use single factor experiment and orthogonal experiment to optimize the water extraction process. Optimum process is12times the volume of solvent, extracting3times, each time1hour. We mix water extract solution with the above, filtration, drying, then get extract powder.Take Periplaneta Americana extract powder as intermediates.Then we research on the preliminary quality standards of intermediates, Character description, Powder properties (Angle of repose and bulk density, tap density, compression index, the critical relative humidity, moisture absorption rate), the determination content of protein and carbohydrate, moisture content, and the study of the extract.Through single factor experiment, we investigate the type of supplementary material filling agent, disintegrating agent and the joining way of powder silica gel. We determine to selects the starch, sodium carboxymethyl starch (CMS-Na), Micro powder silica gel to join way choose to join after grinding method. On the basis of that, mixture design is used to determine the preparation process of the Periplaneta Americana. Medicinal herbs extract powder is39%, starch is39%, CMS-Na is5.2%, the micro powder silica gel is7.4%. The disintegration time is about28.67min. We study the powder property of prescription granules, angle of repose and bulk density, tap density, compression index, the critical relative humidity, moisture absorption rate, to provide the reference for the research of preparation.Preliminary research by Periplaneta Americana chip quality standards, stipulate product properties. We have carried on the preliminary identification. We check weight variation, disintegration time limit, and so on. Using ultraviolet spectrophotometry method, the major effective components content determination of protein, carbohydrate are determinated, and we inspecte the System methodology for determination method.