Based On Reduceing The Toxicity Of Periplaneta Americana To Oil Cream Processing Research

Periplaneta Americana to insecta pterygota blatt hkust germanica insects, beginning in the cold-induced febride, after all previous dynasties materia medica recorded more, today to periplaneta Americana as raw material to develop liver dragon capsule and heart pulse long new liquid injection, rehabilitation, eliminate disease benefit liver slices and other new drugs widely used in clinical, curative effect is remarkable.Periplaneta Americana more than traditional medicine with baked-on after crushing, directly recorded materia medica for toxic or a little poison, in recent years, research shows, periplaneta Americana medicinal powder all have certain side effects on alcoholic liver damage, periplaneta Americana contains palmitic acid, may have a poison effect.Prepared from periplaneta Americana extract some listed items, I also have adverse reactions, but not yet received enough attention.Based on periplaneta Americana side effects as the research point, trying to find out the existence of the toxic effects of site, using processing technology to reduce or eliminate the toxicity of periplaneta Americana, provide guarantee for clinical safe drug use.1 Periplaneta Americana grease acute toxicity studiesThe largest volume 1 and 2 times within 24 h to fill the stomach to periplaneta Americana from different preparation method of grease, observed in mice within 14 d information such as diet, activity, secretion and death and death in mice after gross anatomy observation;Results show that the mice maximum tolerance of periplaneta Americana grease of 1.92 kg of crude drugs. Kg-1,2.44 kg of crude drugs. Kg-1, the equivalent of 60 adult dose of 5 g/kg 384,488 times, explain periplaneta Americana grease has no obvious acute toxicity reaction, prove periplaneta Americana grease is safe and nontoxic to normal mice.2 Periplaneta Americana grease on the model animal toxicity studiesBy intraperitoneal injection of 0.2% CCL4 oil solution caused acute liver injury in mice, the determination of different dose groups of mice aspartic acid transaminase (AST) in serum and the activity of alanine transaminase (ALT) and liver weight coefficient.Results show that the periplaneta Americana grease separate dosing, low, medium and high dose group of liver injury in mice with liver protection and even had a tendency to aggravate liver damage, show the toxic effects;Periplaneta Americana extract oil and merge, periplaneta Americana grease inhibit CCl4 hepatic damage in mice liver weight coefficient of lower (P< 0.01), inhibit mice serum AST, ALT decrease (P< 0.01), after the merger to periplaneta Americana grease inhibit periplaneta Americana extract not only protect liver damage pharmacological effects, even aggravate the model animal liver damage, show the toxic effects.Thus the periplaneta Americana grease exist toxic effects on liver injury model mice.3 The periplaneta Americana attenuated TCM researchAdopt to oil removal of periplaneta Americana oils by frost, acute liver injury model induced by CCL4 in mice were used to investigate periplaneta Americana frost effect.Results show that the periplaneta Americana frost promote CCL4 hepatic damage in mice liver weight coefficient of lower (P< 0.01), and to promote liver injury in mice serum AST, ALT decrease (P< 0.01), suggesting periplaneta Americana frost protective liver damage effect;Periplaneta Americana through to oil cream, eliminate the toxic effect, achieve the purpose of processing attenuated.4 Periplaneta Americana medicine cream process researchUsing different method and the orthogonal experiment design method to oil, periplaneta Americana grease content as indexes, screened periplaneta Americana to oil the best method for oil cream method, optimal degreasing frost processes for crushing five times, each time with 30 mpa pressure squeeze 0.5 h, and the process is stable, reliable and reproducible.5 Periplaneta Americana main ingredients comparison before and after processingThrough the determination of periplaneta Americana extract content before and after processing, oil content, total amino acid content, total sugar content, compared before and after the main ingredients of periplaneta Americana processing changes.Results show that the periplaneta Americana after making cream, extract content increased slightly, significantly reduce the oil content, total amino acids content and total sugar content were increased.6 Periplaneta Americana frost effect validation studiesBy adopting the system of oil cream process after the optimization process from periplaneta Americana cream as raw material, acute liver injury model induced by CCL4 in mice were used to investigate periplaneta Americana frost effect.Results show that the periplaneta Americana frost different dose groups all showed promote CCL4 hepatic damage in mice liver weight coefficient of lower (P< 0.01), and promote the mice serum AST, ALT decrease (P< 0.01), suggesting the periplaneta Americana cream protective efficacy of liver and validation system of periplaneta Americana through to oil cream to eliminate the toxic effect, achieve the purpose of processing attenuated.7 Periplaneta Americana cream quality standards researchBased on ten batches periplaneta Americana raw medicinal materials to oil cream, determination of periplaneta Americana frost moisture, ash content, acid insoluble ash content, extract, oil content, total sugar content, total amino acid content, initially set periplaneta Americana cream quality standards.