Experimental Study And Cinical Effect Observation On Expression Of TXB₂,PGE₂, VEGF And BFGF By Huanglianwendan Decoction Treating Gastric Ulcer（Dampness-Heat Syndrome）

ObjectiveIn this paper,through the establishment of damp-heat syndrome animal model of gastric ulcer,pathological changes,serum markers,immunohistochemical indicators observed after administration in rats,And the effect of berberine Wendantang ulcer patients before and after treatment comparison of clinical symptoms,Discuss the efficacy of gastric ulcer.Methods1.Animal experimentation:Select SD rats90,Male and female,Randomly divided into six groups, each15,there are①control group;②model group;③medicine group;④Chinese high-dose group;⑤medicine dose group;⑥low dose group.In addition to the control group, referring Lvguan Hua, Chen Lu and other heat build ulcer syndrome model. Experimental animals were sacrificed2weeks after femoral artery blood detection TXB2, PGE2levels. Caesarean Section gastric ulcers in rats to measure the area and take the ulcer scar tissue biopsy for HE staining. Detection VEGF, bFGF by immunohistochemistry. Using statistical software SPSS17.0The data were processed, analyzed the results and draw conclusions experiments.2.Clinical observation:Through the clinic met the inclusion criteria,30patients,Were given huanglianwendan decoction for treatment,3weeks for a course,The presence or absence of symptoms observed in patients with significant improvement, with or without statistical significance.Results1.Pathology in rats:Compared with the model group, the experimental groups can reduce ulcer area (P<0.05or P<0.01), Chinese high-dose group was significantly better than western medicine group (P<0.05).Compared with the model group, berberine Wendantang can reduce gastric ulcers in rats score, inflammatory cell infiltration score, fibrosis score (P <0.05or P<0.01), improved ulcer granulation tissue score (P<0.05or P<0.01).2. Effects on rat serum PGE2, TXB2content:Compared with the model group, high medicine, medium and low dose group was significantly higher serum PGE2levels (P <0.05).Serum TXB2were significantly lower (P<0.05or P<0.01).Compared with the model group, western medicine group also raised PGE2levels (P<0.05), reduced TXB2levels (P0.05).3. Stomach tissue VEGF, and bFGF expression:Compared with the model group, berberine Wendantang each group can significantly increase VEGF, bFGF expression (P <0.05or P<0.01).WM group also upregulated VEGF, bFGF’(P<0.01).4.Clinical observation:By huanglianwendan Treating gastric ulcer damp-,After treatment the patient symptom scores decreased significantly (P<0.05),The total effective rate was86.67%,And adverse reactions did not occur during treatment.Conclusion 1.Obtained by experimental study,Huanglianwendan soup can promote healing of ulcers,Mechanism may enhance PGE2, VEGF, bFGF levels mucosal protective factors and promote epithelial regeneration, repair and reduced mucosal aggressive factors TXB2levels and reduce the local inflammatory reaction.2.By huanglianwendan Treating patients with gastric ulcer hygropyrexia,The significant improvement in order to prove the efficacy of clinical symptoms in patients Points,And no adverse reactions...