Correlation Analysis Of Chromosomal Abnormalities And Prognosis In 68 Patients With Multiple Myeloma

Objective:To discuss the relationship between chromosome abnormality and disease progression and prognosis in multiple myeloma.Methods:Through the 68 cases of newly diagnosed multiple myeloma patients underwent conventional cytogenetic analysis(CCA),and application of combined MM probes in fluorescence in situ hybridization(FISH),understanding the relationship between chromosomal abnormalities and progression free survival(PFS) and overall survival(OS).Results:CCA detected 14 cases of abnormal karyotype (20.6%), including 11 cases with complex karyotype,Abnormal karyotype was significantly shorter than normal karyotypes in median PFS and median OS,complex karyotype compared with non-complex karyotype also significantly shorter in median PFS and median OS,the differences were statistically significant (P<0.05).FISH detected 30 cases of genetic abnormalities (44.1%),The abnormal detection rate was significantly higher than that of CCA(χ2 =7.340,P<0.05),The positive rate of 1q21 amplification was 23.5%(16/68),RB1 deletion was 32.4%(22/68),D13S319 deletion was 25%(17/68),IgH rearrangement was 23.5% (16/68),and p53 deletion was 7.4%(5/68).The involving one kind of probe abnormal in 8 cases,involving 2 kinds of probes abnormal in 10 cases, involving 3 kinds,4 kinds and 4 kinds of probes abnormal each of 4 cases.1q21,RB1,IgH and p53 gene abnormalities median PFS,OS was significantly shorter than normal (P<0.05),D13S319 deletion positive than negative patients the median OS shortening, But there was no significant statistical difference (P>0.05).Patients with 3,4 and 5 kinds of probes abnormal the median PFS,OS was significantly shorter than the patients with 1 and 2 kinds(P=0.007 and P=0.018).Comparisons between groups in PFS,OS by ISS staging method,Stage I>Stage Ⅱ>StageⅢ.The median PFS of patients in stage I obviously shorter than that in stageⅢ (P=0.007).However,the median OS comparisons between groups showed no significant difference in Statistics (P>0.05).Conclusion:CCA and FISH detection can be complementary,Simultaneously CCA and FISH can improve the detection rate of chromosomal abnormalities in patients with MM.lq21, RBI, IgH and P53 gene positive patients PFS, OS shorter than the normal. Patients with complex karyotype indicates a poor prognosis.The prognosis of patients with the same ISS stage of heterogeneity,prognostic risk assessment should be comprehensive considered cytogenetics changes and biological indicators.