Study The Structural Reconstruction Of Basilar Artery Wall After Subarachnoid Hemorrhage In Rabbit Model

Objective: To study the pathological changes of the structural reconstruction of basilar artery wall by establishing an experimental model of subarachnoid hemorrhage in New Zealand big ear rabbits used by “Twice injection of blood into the basal cistern ”,and for further studies of CVS pathogenesis.Methods: According to random number table,46 rabbits were divided randomly into two groups :sham operation group(n=10) and experience group(n=36).The experience group is divided into 6 teams(1 day before the operating(-1d),1d,4d,7d,14 d and 21d(n=6 for each)),the sham operation is divided into 5 teams(1d,4d,7d,14 d,21d(n=2for each)).The animal model was induced by twice injections into the basal cistern.Two injections of blood(sham operation group injected with normal saline) 48 hours interval. Removed the basilar artery specimens after perfusion and fixation at different time points. By gradient ethanol dehydration, paraffin embedding, slicing, repair block after HE staining and Masson’s staining. The changes of the vessel wall thickness and the basilar artery lumen diameter were seen under the light microscope, and the data was measured by image analysis system.Results: The rabbit model of subarachnoid hemorrhage was established with low mortality. The luminal diameter of basilar artery was significantly shorter, and wallthickness was significantly thicker, and I/M was larger in experience group 4,7,14,21 days after SAH than those in sham operation group, 1 day before the operating team and 1day after SAH team.Conclusions: “Twice injection of blood into the basal cistern” is a reliable method for establishing model of CVS in rabbits, which can simulate the pathological changes in human who have SAH, and confirmed the existence of vascular injury and inflammation reaction in the process of SAH in cerebral vascular wall reconstruction.