The Expression And Significance Of NOD2m RNA In Peripheral Blood Mononuclear Cells In Patients With Chronic Obstructive Pulmonary Diseases

Objective: Through studying NOD2 m RNA expression in the peripheral blood mononuclear cell nucleus and IL-6 concentration in serum, explore correlation between lung function and NOD2 m RNA expression in the peripheral blood mononuclear cell nuclear and IL-6 concentration in serum of different groups of patients and investigate the role of NOD2 in inflammatory mechanisms and clinical significance for patients with COPD.Methods: Choose outpatient and remission inpatient with COPD in North Sichuan Medical College Affiliated Hospital Respiratory Medicine from October in 2013 to April in 2014. According to pulmonary function testing(FEV1/FVC%、FEV1 predicted%), symptoms(m MRC dyspnea index), a comprehensive assessment of exacerbations in the past year, patients were divided into A, B, C, D four groups, 30 people in each group. 30 healthy examiners in the outpatient on the corresponding period were chosen as healthy control group. Collect peripheral blood samples of patients and the control group, measure NOD2 m RNA expression in mononuclear cell nucleus by real-time fluorescent quantitative polymerase chain reaction(PCR) and IL-6 concentration in samples by enzyme-linked immunosorbent method(ELISA). Analyzing the change of NOD2 m RNA expression of patients with COPD and IL-6 concentration in different groups, explore severity relationship between COPD patients with NOD2 m RNA expression and concentration of IL-6 in different grouping and their correlation.Result:①NOD2 m RNA in peripheral blood mononuclear cell nucleus of COPD stable patients and healthy control group were 0.31 ± 0.18,0.10 ± 0.07, COPD stable group was significantly higher than the healthy control group, the difference was statistically significant(P<0.01); IL-6 concentration in stable COPD patients and healthy controls serum was 10.62 ± 5.01 pg / ml, 3.00 ± 0.40 pg / ml, COPD stable group was significantly higher than the healthy control group, the difference was statistically significant(P< 0.01).②NOD2 m RNA in peripheral blood mononuclear cell nucleus of COPD patient A, B, C, D each group and healthy control group were 0.18 ± 0.10,0.18 ± 0.08,0.36 ± 0.11,0.51 ± 0.17,0.10 ± 0.07, comparing A, B, C, D group and the healthy control group, the difference was statistically significant(P<0.01). Comparing COPD patient A, B, C, D group, NOD2 m RNA expression of D group was significantly higher than group C, the difference was statistically significant(P<0.01), NOD2 m RNA expression of group C was significantly higher than that in group A and group B, the difference was statistically significant(P<0.01), the difference between group A and group B was not statistically significant(P>0.05).③IL-6 concentration in serum of COPD patients of A, B, C, D each group and healthy control group were 6.36 ± 1.82 pg / ml, 6.52 ± 1.97 pg / ml, 12.12 ± 1.78 pg / ml, 17.50 ± 2.22 pg / ml, 3.00 ± 0.40 pg / ml, comparing A, B, C, D group and the healthy control group, the difference was statistically significant(P<0.01). Comparisons between A, B, C, D groups of patients with COPD, IL-6 concentration of D group was significantly higher than group C, the difference was statistically significant(P<0.01), IL-6 concentration of C group was significantly higher than group A and group B, the difference was statistically significant(P<0.01), the difference between group A and group B was not statistically significant(P>0.05).④COPD each group NOD2 m RNA expression in peripheral blood mononuclear cell nucleus of COPD each group increased with IL-6 concentration in serum raising, a positive correlation, the correlation coefficient r=0.82, the correlation was statistically significant(P<0.01). NOD2 m RNA expression in stable COPD patients has a negative correlation with FEV1 predicted%, the correlation coefficient r=-0.85, the correlation was statistically significant(P<0.01). IL-6 concentrations in stable COPD patients has a negative correlation with FEV1 predicted%, the correlation coefficient r=-0.74, the correlation was statistically significant(P<0.01).Conclusions:①NOD2 receptor NOD2 m RNA expression and IL-6 concentration of COPD patient was significantly higher than those of healthy control groups, suggesting that NOD2 and IL-6 involved in COPD occurrence and development.②With COPD exacerbations, NOD2 m RNA expression and IL-6 concentrations gradually increased, NOD2 m RNA and IL-6 might be indicators of COPD severity assessment.③NOD2 m RNA expression and IL-6 concentrations were positively correlated in each group of COPD patients, both of which may interact to promote inflammation.