| Objective(1) To explore the effect of massage to accelerate free radicalscavenging in muscle damage repair process by detecting the energy valueof glutathione peroxidase (GSH-Px), myeloperoxidase (MPO) and theexpression of cellular phospholipase A2(PLA2) mRNA on the repairingprocess after the skeletal muscle injured so as to explore the potentialmechanism of massage on the damage repair.(2) Detecting the expressionof myogenic regulatory factors (Myf5mRNA and MyoD mRNA), as wellas the effects of VEGF (Vascular endothelial growth factor) to investigatethe mechanism of massage to promote muscle fiber regeneration bystudying on the effect of massage on rabbit quadriceps injury.MethodsThe42healthy adult New Zealand white rabbits weighing (2.0±0.5)kg were randomly divided into six groups: the normal control group (A, n=3), the injury group (B group, n=3), the massage group (C group, n=9)and natural recovery group (D group, n=9) after injury5d, the massage group (E, n=9) and natural recovery group (F, n=9) after injury10d. Theexperimental animals of A group were without any treatment, as thenormal controls; the remaining experimental animals of five groups wereprepared rabbit model of right hind limb quadriceps injury blow with ahomemade device. C and E groups began to massage in the3th days aftermodeling, one time per day (2600rpm,15min), until the sacrificing uptime; D and F groups were without massage therapy. All groups’ rabbitsdid ultrasonography at each time period after injury. The samples whichwere taken at the5th day and10the day after injury were respectivelyperformed H&E staining, RT-PCR and Westen blot detection. HE stainingand Ultrasound imaging were employed to inspect pathological changesand tissue necrosis situation of each groups. The SYBR Green real-timequantitative RT-PCR method was employed to detect PLA2mRNA,Myf5mRNA and MyoD mRNA expressions; Western blot was detectedthe expression of VEGF.ResultsAll animals were survived until the completion time of theexperiment. After massage intervention, H&E and Ultrasonographyshowed that the inflammatory response in massage group comparing withthe natural recovery was not obvious, the gap of muscle fibers becomessmaller, and arranged more gradually after injury; the necrosis range wasdecreased, there was angiogenesis in the internal, the density and brightness around the microbubble were higher than the natural recoverygroup. The level of VEGF expression in massage group was significantlymore than the natural recovery group (P <0.01). The activity values ofGSH-Px and MPO were significantly higher in natural recovery groupthan massage group (P<0.05). On the contrary, the expression of PLA2mRNA was decreased in massage group than natural recovery group(P<0.05). In each groups, the myogenic regulatory factor Myf5mRNAexpression were (7.82±0.19),(4.99±0.17),(5.28±0.15),(5.78±0.04),(5.95±0.16),(6.26±0.26), and MyoD mRNA expression were respectively(14.94±0.12),(12.12±0.03),(12.52±0.12),(12.88±0.14),(13.28±0.02),(13.81±0.17), both of the expressions were increased significantly (P<0.01)comparing massage group with natural recovery group.ConclusionMassage can effectively promote the synthesis of muscle collagenfibers, prevent the muscle atrophy, necrosis, and angiogenesis while themuscle damage repair, which will help to repair damaged tissue. Themechanism could be a relationship with that massage can increase theexpression of VEGF, promote the repair of skeletal muscle injury regionalblood supply; accelerate the removal of free radicals, and decreased theactivity of PLA2to protect the structural integrity of the cells, reducepain; promote the expression of myogenic regulatory factor Myf5 mRNA and MyoD mRNA which is Conducive to the generation ofskeletal muscle fibers. |
PDF Full Text Request