Research Separation And Purification Of Aucubin From Eucommia Seed Meal And Eucommia Seed Meal’s Chemical Composition

Eucommia ulmoides Oliv. is chinese precious ancient tree species, whoes contains a variety of natural active ingredients, is Chinese unique precious tonic herbs. Its medicinal has a long history and has been widely used in clinical application. Eucommia skin is a traditional Chinese medicine, but recent studies have found that Eucommia’s skin and leaves contain similar active ingredients.so we can consider that Eucommia leaves is the medicine like Eucommia skin. Eucommia seed, flowers also have high medicinal value. Eucommia also the greatest development potential source of high quality rubber tree. Eucommia contains a lot of white filamentous gutta-percha, the quality is better than traditional rubber, widely used in the aerospace, defense, industrial rubber, chemical, medical, and ships. In the plant,Eucommia ulmoides seed contains a lot of aucubin as high as11%. It is worth further research and development and utilization.The seed draff of Eucommia is the things that after cold rolling grease from Eucommia ulmoides seed,and which is rich in Aucubin. Today Eucommia seed only used as a feed additive, its medicinal value is far from exploitation.1. AU of the seed draff of Eucommia ulmoides Oliv. was determined by Reversed Phase-High Perfomance Liquid Chromatography (RP-HPLC). The chromatographic conditions are as follows:column: Thermo Hypersil Gold C18(250mmx4.6mm,5μm), mobile phase:methanol-water (8:92), detection wavelength:206nm, flow rate:1mL/min, column temperature:25℃. After a methodological study, this method is reliable for the determination of Aucubin of the seed draff of Eucommia ulmoides Oliv., and the results of Aucubin content is15%.2. Separation and Purification of Aucubin from Eucommia ulmoides SeedMeal By macroporous resin. After a single factor tests between Loading capacity, eluent on the macroporous resin. Selected the best purification process of AU from Eucommia ulmoides seed meal by H103macroporous resin. Through this process, we can get about a purity of45%Aucubin from Eucommia ulmoides seed meal by H103macroporous resin.3. Separation and Purification of Aucubin from Eucommia ulmoides seed meal By silica gel normal column chromatography. After a single factor tests with the45%Aucubin from Eucommia ulmoides seed meal between Loading capacity, eluent on the macroporous resin. Selected the best purification process of AU from Eucommia ulmoides seed meal by silica gel normal column chromatography. Through this process, we can get about a purity of78%Aucubin from Eucommia ulmoides seed meal.Then through Reversed phase silica gel column chromatography and Preparative high performance liquid chromatography we can get about higher than98%of the Aucubin from Eucommia ulmoides seed meal.4. Studies the chemical composition on Eucommia ulmoides seed meal. Through the Reversed phase silica gel column chromatography,Preparative high performance liquid chromatography and macroporous resin. We get six compounds from Eucommia ulmoides seed meal. These compounds were identified by13C-NMR,1H-NMR, ESI-MS.They are Glucose, Dibutyl Phthalate, Ursolic acid, Aucubin, Betulinic acid, Betulinol.