| Background Significant impact of depression on health, depression often depressed, pessimistic. Severe depression, suicide rates up to about15%, unexplained mood for those who pay attention to their sluggish behavior early, early prevention, and medicine have a certain effect for the treatment and prevention of depression. Among the many hypotheses in the pathogenesis of depression,"monoamine hypothesis" in antidepressant research and development is still in a dominant position. Hot research topic in recent years in the search for low toxicity, high efficacy of antidepressant drugs. LNT able to modulate the immune system, boost the immune cell maturation, differentiation and variety of biological effects of anti-tumor, anti-oxidation, anti-aging, lowering blood sugar. The experiments to further explore the mechanism of action of lentinan (Lentinan, LNT) antidepressant.Objective:By observing the hippocampus5-HT levels and5-HT1A receptor expression, MDA content and SOD activity and the effect of lentinan mouse model of chronic stress behavior. To investigate the mechanism of antidepressant LNT.Method:Healthy adult mice were50,♂, body weight (20±2g), SPF grade, based on1%sucrose water partial addicted degrees and were randomly divided into four groups, namely, normal control group (Normal controls, NG), model in the control group (Model control, MG), LNT dose group (L-LNT,2.5mg/kg; H-LNT,5.0mg/kg). Before the experiment began modeling1h daily oral administration, continuous administration28d, each experimental group administered according1.0mL/100g weight. Application chronic unpredictable mild stress model of depression produced, and make improvements. Normal control group, not to stimulate, food and water properly. Model group and the experimental intervention group with the lone support, fasting, water deprivation (24h), and accept the unpredictable stressors, the28d of the experiment, the animals were randomized to receive daily each only one stimulus, within five days after making the choreography the stimulus program is not repeated and unpredictable. Animals were observed daily behavior change, with a strange environment to start feeding feeding experimentally observed incubation period, forced swim stress test record swim immobility time, enzyme-linked immunosorbent assay of hippocampal5-HT content, NBT assay of total SOD activity, thiobarbituric acid (thiobarbituric acid, TBA) MDA content assay, immune proteins mark (western blot) to detect5-HT1A receptor expression levels.Results:MG mice in an unfamiliar environment, feeding latency was significantly prolonged after giving LNT intervention can shorten lead to chronic stress in mice feeding latency in an unfamiliar environment, prolonged chronic stress leads to significantly reduce the stress in mice swimming in water immobility time extension,5-HT levels and5-HT1A receptor expression enhancing, LNT intervention group increased SOD, MDA content decreased and Antagonize TNF-a and IL-6expression in immune regulation function, compared with the model group MG indicators above improvements were statistically significant (P <0.05orP<0.01).Conclusion:①LNT can significantly antagonize depressive symptoms in mouse models of chronic stress, increased locomotor activity in mice time;②LNT increase in hippocampal5-HT levels and5-HT1A receptor expression and Antagonize TNF-a and IL-6expression in immune regulation function and increase SOD, MDA content may antagonize the antidepressant effects and mechanisms related to LNT. |
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