Extraction Active Components In Cortex Moutan And Research On Hypoglycemic And Antioxidative Bioactivity

diabetes is one of the world’s most common chronic diseases, the research showed that the ROS was related closely to diabetes and its complications,enhanced oxidative stress is a major pathogenesis of chronic complications of diabetes.It has a long history of traditional Chinese medicine,and it also has the advantage therapeutic effects included that the muti-directional,muti-faceted and muti-target. Traditional Chinese medicine has shown the great value and development potential.Cortex moutan is the dry velamen of Paeonia suffruticosa which belonged to Perennial herb of Ranunculaceae.The paper reported the extraction active components in cortex moutan and research on hypoglycemic and antioxidative bioactive. The research results as follow:1、Screening of the commonly used antidiabetic traditional Chinese medicineRetrieve the prescription of the treatment of diabetes and its complications, according to the blood sugar drop、T-SOD、MDA、AR and kidney weight/body weight(%),plus herbs regional,Selecting Chongqing gepherbs cortex moutan as the object of in-depth study, which has better antidiabetic activity than others.2、Extraction and purification of the active position from cortex moutanThe diabetic mice experiment showed that the antidiabetic activity of alcohol extract was better than water extract. Study four extracts and the whole extract to HepG2cell with the subject of consumption glucose affection and to ROS level in cell,select the ethyl acetate phase to be separated.3、Study on the hypoglycemic activity of the active componentsof cortex moutanFour phases all could add glucose consumption in cells.At high concentration, hypoglycemic activity expressed that:aqueous phase>petroleum ether phase>ethyl acetate phase>the whole extract>butanol phase.In the same dose,aqueous phase has larest decline to the blood sugar of the diabetic mice,the whole extract is the lowest.4、Study on the antioxidative activity of the active componentsof cortex moutanAntioxidative activities of the active componentsof cortex moutan were assessed by mitochondria H2O2,O2-, ABTS and ROS level in HepG2cell in vitro.The result showed that the antioxidant activity of ehhyl acetate phase is best;the clearance rate to O2-of four phases is better than to ABTS Free radicals. Methyl gallate has the best clearance of ROS in cells, paeonol as followed, paeoniflorin’s is the lowest.Three compounds all could clear the H2O2in mitochondria,paeonol’s is the highest,methyl gallate as followed,paeoniflorin is the lowest.