Comparative Study Of Pre-and Post-processed Bozhou Cortex Moutan The Pharmacodynamic

Objective:The progesss of the times, the development of technology, the accelerated pace of life, of people’s diet with a huge change in energy intake in the diet increase, the amount of exercise to reduce. The incidence of hypertension, high cholesterol, diabetes, myocardial infarction and other cardiovascular and cerebrovascular disease continue to increase, and there is an increasing trend.Traditional Chinese drugs Cortex Moutan in previous studies has been confirmed to have a better therapeutic effect on diabetes and myocardial ischemia and reperfusion. Herbs of good quality to have a good therapeutic effect, but the poor quality of the medicine only can not play the role of treatment, it will give pain body worse. At present, the Chinese traditional medicine market, there is an unwritten practice of Chinese herbal medicine during conconcted in order to ectend the shelf-life time of the herbs, the majority of businesses will be concocted herbs to add a process-smoked with sulfur.This can make the medicine long-term appearance not only beautiful but also to ensure that the medicine storage time.Bozhou as the Chinese herbal medicines wholesale market in China, bringing together herbal medicines from around the country selling businesses. According to the status of Chinese herbal medicines, I planted by the local purchase in Bozhou, after moutan by sulfur smoked before. The main purpose of this study by sulfur smoked before, after the tree peony bark for the treatment of diabetes mellitus and myocardial ischemia reperfusion injury, observe the existence of a certain influence its officacy after sulfur smoked, and to the extent of the impact a comparision. Experimen to draw more scientific evidenvce to explain the roleof sulfur smoked such a process is necessary.Method:1、The health of SPF male mice adaptive feeding three days, with a homemade high fat diet for4weeks, a one-time to the mice by intraperitoneal injection of STZ solution, resulting in high glucose, glucose≥11.1mmol L-1mice were used as the standard of type Ⅱ diabetes model. The model mice were randomly divided into five groups:normal group, model group, metformin posit ive group, the the Bozhou paeonol group before processing, after processing the Bozhou Cortex Moutan group. Normal mice given a normal diet, the rest of the group is still given high fat diet. Four weeks of continuous administration.4weeks after the determination of the glucose tolerance of mice in each group. Removal of the mouse eye blood, anatomical remove the mouse heart, liver, spleen, lung, kidney, part of the liver, kidney made of biopsy is used for microscopic observation and measurement of serum GLU and, TC, TG and SOD, MDA and NEFA content.2, Will be purchased male Wistar rats after3days of adaptation to the environment randomly divided into5groups:normal group, model group, sham operation group, the Di’aoXinxuekang Kang positive group, the Bozhou Cortex Moutan group (n=10). Intragastrically for14days. After14days, weighing at1h after the last dose, anesthesia, connect the lead II ECG. The middle from the neck skin incision and separation of the trachea, Tracheal intutation, mechanical ventilation to connect small-animal ventilator. In thirty-fourintercostal thoracotomy, the skin incision, blunt dissection of muscle tissue, and open the chest, separating the pericardium, the heart out of chest, left anterior descending artery ligation the heart of the left coronary artery, causing ischemia. The heart back into the chestcavity, unlock ligation, ischemia30min continueperfusion60min. Cutthe abdominal cavity, abdominal aorticblood, take the rat heart, liver, kidney, part of the heart made apoptosisis used for microscopic observation and determination of SOD in serum, MDA, CK of LDH, GSH and NO content.Results:1、The Bozhou paeonol number of micedied after the one from thegavage process given the processing is twice prior to processing. Observed from impaired glucose tolerance results, before processing and after Bozhou paeonol can reduce postprandial blood glucose concentrations, no significant differences between the two. Compaered with model group, pre-and post-processed Bozhou Cortex Moutan group can significantly reduce the type II diabeticmice GLU, TC and MDA, and significantly incereased type II diabeticmice, SOD, and processed prior to processing Bozhou Cortex Moutan TC, TG and SOD, MDA, and NEFA, there was no significant difference,but from the degree of recovery than the paeonol effect after process prior to processing, good.2、Compared with model group, paeonol groups can significantly reduce serum MDA, CK and LDH levels, elevated SOD and GSH activity; the paeonol group serum content of NO although increased, but nosignificant difference compared with model group. HE staining showed:Cortex Moutan myocardialcells compared with model group, arranged in neat rows, and other pathological changeslessened. TUNEL staining results:Compared with model group, paeonol groups can reduce the apoptotic index, and has significant differences.Conclusion:1、The Bozhou Cortex Moutan in mice the number of deaths from the process of intragastric administration, given the processing is twice prior to processing. Observed from impaired glucose tolerance results, pre-and post-processed Bozhou Cortex Moutan can reduce postprandial blood glucose concentrations, and no significant differences between the two. Compared with model group, pre-and post-processed Bozhou Cortex Moutan group can significantly reduce the type II diabetic mice GLU, TC and MDA, and significantly increased type II diabetic mice, SOD, and pre-and post-processed Bozhou Cortex Moutan group for TC, TG and SOD, MDA, and NEFA, there was no significant difference, but from the degree of recovery, effect for Bozhou Cortex Moutan of processing before is more excellent than the thepaeonol after processing prior to processing.2、The Bozhou Cortex Moutan experimental myocardial ischemia reperfusion injury protection mechanism may be related to scavenging oxygen free radicals, protect the oxygen free radical scavenging Enzymes, to enhanceischemia-reperfusion in rat myocardial antioxidant capacity and inhibition of lipid peroxidesproduction, reduce the lipid oxidation damage of induced myocardial cell membrane; and increase endogenous NO production, expansion of coronary microcirculation, which has protective effects on myocardial cells.