| Purpose:Observing the curative effects of "through moxibustion" and moxibustiontreatment of renal colic and the effect of "through moxibustion" pretreatment onkidney stones model rats. In the animal experimental aspect, the work researchdifferent moxibustion on the analgesic effect of rat model of renal colic, put thechange of the content of5-HT, β-EP in the thalamus and hypothalamus; calcium (ca2+)level changes in rats urine and blood; Cr and BUN levels change in the rats blood; therats pain reaction time changes as indicators, assess the effects of dfferentmoxibustion, research analgesic action of different moxibustion to rat model of renalcolic, establishing optimal treatment effect of moxibustion，expound the mechanism,and guide clinical practice.Materials and methods:This experiment selected40male rats, were randomly divided into control group(A group), model group (B group),"prevention moxibustion" group (C group),"normal moxibustion" group (D group),"through moxibustion" group (E group), thereare8rats in each group. Rats were fed adaptively a week. On the eighth day of theexperiment,"prevention moxibustion" group (C group) began pretreatment.On the15thday of the experiment,except control group, the rats of other groups drank thebuilt model liquid (1%Ammonium chloride and1%Ethylene glycol mixed liquid)freely. On the21stday of the experiment, D group and E group started treatment, Cgroup began build model, in the process of build model and treatment, observed therats’ spontaneous pain behavior."Prevention moxibustion" group (C group),"normal moxibustion" group (Dgroup),"through moxibustion" group (E group) choose shenshu point duringtreatment, moxibustion method combine bird-pecking moxibustion and circlingmoxibustion, make the skin flush, no burns as moderate. C group begin pretreatmentfrom the8THday of experiment, the pretreatment use "through moxibustion", that moxibustion for11-13minutes a pretreatment,7days. D group use "normalmoxibustion", the treatment began from the21stday of experiment,5minutes for eachtreatment, last7days. E group use "through moxibustion", that moxibustion for11-13minutes each treatment,7days in total. In the28stday of the experiment, aftertreatment5minutes, measure every rat pain reaction time by "Hot sting apparatus".Before the end of the experiment1day, use metabolic cages to collect all rats’ urine24h, to detect calcium (Ca2+) level in the urine. One day after the experiment,8:00AM,separately inject each group rats20%urethane, after anesthesia, draw blood fromabdominal aorta5ml, add anticoagulant, measuring serum calcium (Ca2+), Cr, BUNlevels. Pick each rats’ thalamus and hypothalamus, detect the content of5-HT andβ-EP in them. The physical and chemical testing above use ELASA method todetected.Results:1. The content of5-HT and β-EP of thalamus and hypothalamus in rats:Content of5-HT: compared with A group: B group5-HT content lower, havedifference (p<0.05); E group5-HT content rise, have difference (p<0.05); C groupand D group have no difference (p>0.05). Compared with B group: C group5-HTcontent rise, have difference (p<0.05); D group and E group5-HT content rise, havestatistical significant difference (P<0.01). Compared with C group: D group have nodifference (p>0.05); E group5-HT content rise, have difference (p<0.05). D groupand E group have no difference (p>0.05).Content of β-EP: compared with A group: B group, D group, E group β-EPconten rise, have statistical significant difference (P<0.01). Compared with B group:D group, E group β-EP conten rise, have statistical significant difference (P<0.01).Compared with C group: D group, E group β-EP conten rise, have statisticalsignificant difference (P<0.01). Compared with D group: E group β-EP conten rise,have statistical significant difference (P<0.01).2. Rats pain reaction time after treatment:Compared with A group: B group pain reaction time lower, have statisticalsignificant difference (P<0.01); C group and D group have no difference (p>0.05). E group pain reaction time rise, have statistical significant difference (P<0.01).Compared with B group: C group, D group and E group pain reaction time rise, havestatistical significant difference (P<0.01). Compared with C group: D group have nodifference (p>0.05); E group pain reaction time rise, have statistical significantdifference (P<0.01). Compared with D group: E group pain reaction time rise, havestatistical significant difference (P<0.01).3. Rats blood and urine calcium (Ca2+) level measurement:Rats blood calcium (Ca2+) level measurement: compared with A group: B group,C group, D group and E group blood calcium (Ca2+) concentration rise, havestatistical significant difference (P<0.01). Compared with B group: C group, D groupand E group blood calcium (Ca2+) concentration lower, have statistical significantdifference (P<0.01). Compared with C group: D group and E group have nodifference (p>0.05). D group and E group have no difference (p>0.05).Rats urine calcium (Ca2+) level measurement: compared with A group: B groupand C group urine calcium (Ca2+) concentration rise, have statistical significantdifference (P<0.01); D group and E group have no difference (p>0.05). Comparedwith B group: C group have no difference (p>0.05); D group and E group urinecalcium (Ca2+) concentration lower, have statistical significant difference (P<0.01).Compared with C group: D group and E group urine calcium (Ca2+) concentrationlower, have statistical significant difference (P<0.01). D group and E group have nodifference (p>0.05).4. Rats blood Cr, BUN level measurement:Rats blood Cr level measurement: compared with A group: B group, C group andD group blood Cr concentration rise, have statistical significant difference (P<0.01); Egroup have no difference (p>0.05). Compared with B group: C group have nodifference (p>0.05); D group and E group blood Cr concentration lower, havestatistical significant difference (P<0.01). Compared with C group: D group and Egroup blood Cr concentration lower, have statistical significant difference (P<0.01).Compared with D group: E group blood Cr concentration lower, have statisticalsignificant difference (P<0.01). Rats blood BUN level measurement: compared with A group: B group, C groupD group and E group blood BUN concentration rise, have statistical significantdifference (P<0.01). Compared with B group: C group, D group and E group BUNconcentration lower, have statistical significant difference (P<0.01). Compared with Cgroup: D group have no difference (p>0.05); E group BUN concentration lower, havestatistical significant difference (P<0.01). Compared with D group: E group BUNconcentration lower, have statistical significant difference (P<0.01).5. Spontaneous pain behavior score (see scoring method Attal, etc.) were:After building model, B group, D group and E group spontaneous pain behaviorscore showed no difference (p>0.05); after treatment, D group and E grouprespectively compared with B group have statistical significant difference (P<0.01); Dgroup compared with E group have significant difference (p<0.01).Conclusion:1."Through moxibustion" and "normal moxibustion" can affect the change of thecontent of5-HT, β-EP in the thalamus and hypothalamus of rat model of Calciumoxalate stone renal colic, the change of pain reaction time, blood and urine calcium(Ca2+) level, blood Cr, BUN level, Spontaneous pain behavior score,"throughmoxibustion" and "normal moxibustion" have effect on rat Calcium oxalate stone inrenal colic.2. The different effect of moxibustion,"through moxibustion" rise more rat β-EPcontent in the brain,rat pain reaction time; reduce more rat blood Cr and BUNconcentration than "normal moxibustion".3. The effect of "Through moxibustion" pretreatment on kidney stones modelrats,"Through moxibustion" pretreatment reduced the concentration of BUN and Ca2+in blood, and had interference effect on the formation of kidney stones.4. Analgesic action mechanism of "through moxibustion" on rat Calcium oxalatestone renal colic: improve the content of5-HT, β-EP in the rats thalamus andhypothalamus, occurred analgesic action, improve pain reaction time of rat. |
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