Experimental Research On The Skin Aging Mice Model Induced By D-Galactose Of Qiyuan Plaster And Erhuang Capsuie

Objective: To investigate anti-aging effect and mechanism of Qiyuan plaster and Erhuangcapsule on aged mice induced by D-galactose. Method: Choose 50 three months mice andrandomly divide into 5 groups: Qiyuan plaster dose group, Erhuang capsule dose group,blank control group, model control group and vitamin E group. Mice blank control groupreceived subcutaneous injection on the back of neck with physiological saline, and mice inother 4 groups received subcutaneous injection of D-galactose (1000mg-kg-1·d-1.m) for42d to establish subacute aging model.After 22d, Qiyuan plaster-treated (200mg.d-1.gastricinjection) group, Erhuang capsule-treated (200 mg.kg-1·d-1, gastric injection) group,and vitamin E-treated (50 mg·d-1, gastric injection) group, blank control group andmodel control-treated (0.2ml.d. physiological saline, gastric injection) group, To calculatethe statistical after 22 d. the dorsal skin samples were collected to determine SOD、CATactivities and MDA、GSH-PX、hydroxyproline contents, and to calculate and analyze thedepth of surface tissue and dermis tissue from the back neck of mice withphotographs-analyzing system of computer. Result: 1. There was a significant differencebetween blank contol groups and model control group (p＜0.05 or p＜0.01). 2. The erhuangcapsule group and the Qiyuan plaster group can reduce the MDA content induced byD-galactose in mice’s the blood, but the effect was not significant. 3. The erhuang capsulegroup can obviously enhance senile model induced by D-galactose mice’s blood serumSOD activeness (P＜0.01), but compares with the other control groups, the Qiyuancapsule group’s the effect was not significant. 4. The erhuang capsule and the Qiyuanplaster can obviously enhance the depth of epithelium and dermis of sub-senile mice’smodel induced by D-galactose (P＜0.01). 5. There was no significant difference betweenthe erhuang capsule group and the Qiyuan plaster group (p＞0.05). Conelusion: Qiyuanplaster and Erhuang capsule has good anti-aging effects on the aged mice, which isprobably due to effects of increasing the content and activist of epithelium and dennistissue, and the influence of increasing depth of the epithelium and dermis tissue, displaying the progressing of degeneration of elastic fiber and collagen fiber of the tissues and organsto drop.