The Expression Of Activin Receptors In Placenta And Their Relationship With FGR

Objective:To detect the localization and expression of activin receptors in placental tissue offetal growth restriction(FGR) and normal term pregnancy,to investigate their relationshipwith FGR,and to explore the roles of activin receptors on the pathogenesis of FGR.Methods:Thirty cases of placenta were collected from pregnent women in obstetricaldepartment of our hospital,which including FGR and normal term pregnancy respectivelyfifteen cases.The placental samples were detected by HE stain,and the localization andexpression of activin receptors were analysed by immunohistochemistry.Real-Time PCRwas used to detect the expression of activin receptors genes.Results:1. HE staining results: in control group,villi developing well,blood vessels expandingdistinctly,there were less interstitial cells and syncytiotrophoblast predominated thanFGR group.Besides,there were more interstitial fibrosis, calcification and necrosis ofvilli in FGR group.2. Immunohistochemical staining results: activin receptors of two groups both expressmostly in the cytoplasm of syncytiotrophoblast and trophoblast.The degree of positivestain of ActRⅡ A、ActRⅡ B in FGR group was significantly higher than controlgroup(P＜0.05),but the expression of ActRⅠ A、ActRⅠ B was almost the same level intwo groups(P＞0.05).3. Real-Time PCR results: the quantified relative expression of ActRⅡ A、ActRⅡ B genein FGR group was significantly higher than that of control group(P＜0.05),but thequantified relative expression of ActRⅠ A、ActRⅠ B gene was almost the same level in two groups(P＞0.05).Conclusion:1. The expression level of ActRⅡ A、ActRⅡ B in placenta of pregnancy with FGR wasobviously higher than normal term pregnancy,while the expression level of ActRⅠ A、ActRⅠ Bwas almost the same in two groups.2. The expression level rise of ActRⅡ A、ActRⅡ B in placenta of late precnancy may bean important factor which leading to FGR.3. Activin and its receptors were involved in the regulation of fetal growth,the disorder ofactivin receptors may play an important role in the pathogenesis of FGR.