Configuration And Evaluation On Effect Of Anti-a-influenzavirus Of Hand-washing Agent With Heterpolyoxometalates

Objective：Influenza, commonly known as the flu, can cause acute respiratoryinfection. Influenza has some characteristics, such as strong infectivity, rapidtransmission, generally susceptible to populations, etc. The most common symptomsare chills, fever, runny nose, headache, coughing and general discomfort. Flu canoccasionally lead to death. The most recently influenza pandemics occurred in April2009, spreading from Mexico and the United states, quickly crossed the world,resulting in about18000deaths. Influenza epidemic remains one of the serious publichealth problems in China. Typically influenza is transmitted through the air andcontact with nasal secretions or contaminated surfaces. Using hand sanitizer withactive anti-virus ingredient is an effective and workable way to interrupt flutransmission. Polyxoxmetalatesare early transition metal oxygen anion clusters.Studies indicate that most polyxoxmetalates with Keggin structure has broad spectrumanti-RNA and anti-DNA virus activities and low toxicity. Hand sanitizer withpolyxoxmetalates can prevent the spread of the flu. In this paper, hand sanitizers withpolyxoxmetalates was prepared and theirs anti-A-influenza virus effect was evaluated.Methods：1.Thecytotoxicity and anti-virus effect of the two compounds(A and B) wasdetected on MDCK cells.2.The hand sanitizer was synthetized according to generalmethod. Three levels of compound A and B were chosen to add into hand-washingagent.Performance of hand sanitizer was evaluate according to GB19877.1-2005.3.The anti-A-influenza virus effect test was performed according to theAmerican Society for Testing and Materials(ASTM) standard assessment protocols.The natural viability of H1N1on hands for30min and60min without hand washingwas also assessed.4.The hand hygiene with high A was chosen for toxicity assessment using Kunming mouse and rabbits according to Disinfection Technical Specifications(2008).Results:1.The TC50of two compounds (A and B) were2363.12mg/L and1301.25mg/L. The IC50of two compounds (A and B) were28.58mg/L and18.82mg/L.The therapeutic indexes of the two compounds were82.68and69.13.2.Theappearance, color, smell, totally active content, pH value, solid content, foam heightand stability of hand sanitizer conformed to the requirements of the standard.3.TheH1N1concentration in control group was104.29±0.41TCID50/0.1mL. The fingersin treatment groups had no viable H1N1recovered. The virus concentration intreatment group had significant differences compared with the control group(P<0.001).The H1N1virus concentrations in30and60min groupswere103.98±0.42TCID50/0.1mLand103.83±0.35TCID50/0.1mL and had no significant differences compared with controlgroup(P>0.05).4.The LD50of mouse acute toxicity test was>5000mg·kg-1, the intactand damaged rabbit skin irritation indexes were0.33and1.33, and the eyesstimulation indexes of three rabbits were0,0.67and0.33.Conclusion:Hand sanitizer with Heterpolyoxometalates has better anti-A-influenzavirus effect and lower toxicity. It has slight stimulation to skin and eyes of rabbit (nostimulation to intact skin and eyes). This study laid foundation for furtherdevelopment of antiviral hand sanitizers with heterpolyoxometalates in public health.