K-state Of The Ras Genes And Colorectal Cancer Clinical Pathologic Correlation Research

Purpose:1.Detection of colorectal cancer patients in the region K-ras gene mutation, themutation rate to understand, evaluate prospects locally targeted therapy in patients withbiological and make prognosis;2.Combining mutation and postoperative pathology report in detail, betweenclinicopathological analysis of K-ras gene status and whether the association.Methods: ollection this homes (Bengbu medical school first subsidiary hospital)gastrointestinal surgical January2011to November2013during, in this sections lineknot rectal cancer surgery of surgery specimens (each cases patients are take CancerOrganization and cancer next organization little)93cases; line real-time fluorescentquantitative PCR (RT-qPCR)+high tell melt solutions curve analysis (HRMA) check,detection local patients K-ras gene mutations rate, while and pathology check results docontrast, research this area knot rectal cancer K-ras gene mutations ratio And itscomparison between clinical pathology and general information to study both relevancy.Results:1.93cases of successful detection of k-RAS gene, the mutant in34cases (36.56%),exon2of the12and13th codons, mutation rates between27.96%,8.6%, respectively;2. Poorly differentiated type of mutation rate54.28%(19/35,3519cases ofmutations among patients with poorly differentiated, the same below), differentiate into23.68%(nine-thirty eighths), high differentiation for30%(six-twentieths); ⅰ and ⅱin patients with mutation rate20.93%(nine-forty thirds), phase ⅲ52.5%(twentyone-fortieths), phase IV40%(four-tenths) mutation rate or lymph node metastasis in thedistance48.08%(twenty five-fifty seconds), No distance or21.95%lymph nodemetastasis (nine-forty firsts), mutation rates in patients over60years old44.19%(nineteen-forty thirds), the age of60,30%(fifteen-fiftieths), the male mutation rate inpatients with37.74%(twenty-fifty thirds), female mutation rate in patients with35% (fourteen-fortieths) and right colon mutation rate39.28%(eleven-twenty eighths), leftcolon and sigmoid colon mutation rate of29.03%(nine-thirty firsts), Mutation rates inpatients with colorectal cancer at41.18%(fourteen-thirty fourths);3.Using the results of the statistics package SPSS18.0display: high or low degreeof tumor differentiation and Clinical pathological staging different sooner or later, thedifference between K-ras gene mutation rates significantly, there was a statisticallysignificant (P<0.05), K-ras gene mutation rate or not, and the patient’s age, gender,tumor site less relevant (P>0.05).Conclusion:1.Local K-ras gene mutation in patients with colorectal cancer rate is36.56%;2.Mutant K-ras gene are more likely in higher malignancy, the staging of clinicalpathology in colorectal cancer patients with late detection. Has nothing to do withpatient age, gender, tumor site.