Extraction Technology And Bacteriostatic Activity Of Chelerythrine

Chelidonium majus, in which alkaloids as the main active ingredient are of papaveraceae chelidonium. This paper studied the celandine seed processing method:with20%polyethylene glycol (PEG) to soak the seeds for16hours,the highest germination rate was under the condition of12hours light and12hours darkness. And seted up celandine artificial cultivation techniques.Research chelerythrine extraction scheme,the optimized extraction conditions have been founded through the single factor experiment and the orthogonal test.The roots was obtained by75%alcohol with the liquid/solid ratio of1:8for35min extraction under85%of ultrasonic frequency.For the stems, which were obtained by85%alcohol with the liquid/solid ratio of1:8for30min eatraction under75%of ultrasonic frequency.Leaves were obtained by55%alcohol with the liquid/solid ratio of1:10for40min extraction under85%of ultrasonic frequency.Compared the variation of wild and planted celandine plants red alkali. From June to September,the content of artificially cultivated Chelidonium majus roots was higher than that in wild Chelidonium majus. However, the chelerythrine in stems of artificial cultivation was lower than that of the wild, For the leaves, the chelerythrine of the wild was higher than the artificial in June and July, the condition in August and September was opposite.Observed celandine red base for plant disease pathogen inhibition. When the concentration was0.006mg/mL,the chelerythrine extracts displayed a certain inhibitory effect on Bipolaris maydis, Pestalotiopsis sp, Septoria microspora, Fusarium oxysporum f.sp.lycopeersici, Fusarium bulbigenum, Curvularia lunata. Inhibitory effect on Fusarium oxysporum f.sp. lycopeersici best could reach81%, Pestalotiopsis sp inhibition was a bit small, also reached48%. When the concentration was0.014mg/mL, inhibitory effect on Septoria microspora reached96%.For the other five kinds of pathogenic bacteria inhibition rates were above85%.Spore germination experiments deeply researched inhibition of pathogenic bacterias. when chelerythrine diluted10000times inhibition to Septoria microspora spores germination reached47.64%, the inhibition to Curvularia lunata spores was minimum, but also could reach12.05%.Under the effect of concentrate inhibition to Septoria microspora spores strongest reached96.67%, the minimal effect on Curvularia lunata was84.91%. Spore germ tube of control spores was significantly longer than liquid drug.So chelerythrine has the characteristics of less dosage and obvious antibacterial effect, it is the rare source of botanical pesticide development.