| Microfluidic chip is a recently developed new analytical techniques, it is simple,rapid, separation efficiency, low sample or reagent consumption, easy tominiaturization, integration, automation. In drug analysis, life science, environmentalmonitoring and food security and other fields it has shown an important role.Liquid extraction, filtration, non-proliferation and other membrane separationmeans-Microfluidic analysis systems technology into liquid by capillaryelectrophoresis separation from the core analysis. Multiphase flow separation layerwhich is simple microfluidic system architecture, there are several separate functions,with a wide range of applications.Under the background of the requirements of the analysis system in today’smedical field enhances. Microfluidic chips have brought new hopes to thepharmaceutical analysis be more efficient, faster, and lower energy consumption. Inthis work, he applications of microfluidic chip capillary electrophoresis inpharmaceutical analysis were studied. The extraction and separation and detection ofchelerythrine and sanguinarine in Chinese herbal medicines were studied, hoping tobe an effective method for the analysis of traditional Chinese medicine witch combinethe extraction with the separation.In the first chapter, the development of microfluidic chip, with a focus on itsapplications on drug pre-treatment and drug testing in the analysis (analysisof drugs invivo, analysis of chemical medicine and traditional Chinese medicine and theirpreparations, analysis of chiral drugs, and other progress fields of drug research) wasreviewed.In the second chapter, developed a method for the determination of sanguinarinein Chelidonim majus by microchip capillary electrophoresis with laser induced fluorescence detector. By the use of the natural fluorescence of sanguinarine wasincreased by the addition of SDS. Sanguinarine was separated with fluoresceinsodium as an internal standard on microchips in20mmol/L borate-SDS buffersolution(pH=9.0). The concentration and pH value of buffer solution, the additivesand the separation voltage was optimized. Sanguinarine and fluoresecein sodium canbe separated in80s. This method is simple, sensitive, accurate, and it could be usedfor the determination of sanguinarine.In the third chapter, A new method to separate and determine the content ofsanguinarine and chelerythrine in the seeds of Macleaya cordata by nonaqueouscapillary electrophoresis on microfluidic chips with laser induced fluorescence wasdeveloped. In this work, the separation effects of different organic solvents, the pHvalue of buffer, the coated surface of chips bydimethyldichlorosilane was investigated.Ammonium acetate-acetic acid-formamide-water (volume ratio=2:0.5:5:2.5,pH*=5.55) was used as the electrophoresis buffer solution. The separation can befinished in150s. However, when the surface of the channels was coated withdimethyldichlorosilane, the separation can be furtherly improved. The method can beused in the determination of chelerythrine and sanguinarine in the seeds of Macleayacordata. And provides a good method to separate the components with similarstructures.In the fourth chapter, by controlling the flow rate of the liquid injection pump,formed a liquid-liquid two-phases laminar flow in the microfluidic channels, becauseof the molecular diffusion effects between the two liquids in the two-phases interfaceand the difference of solubilityof chelerythrine and sanguinarine in the organic phaseand aqueous phase liquids, the extraction and enrichment of the alkali from theorganic phase to the aqueous wan achieved.In the fifth chapter, a method to separate and determine the content ofsanguinarine and chelerythrine in Chelidonim majus by HPLC was developed.Chromatographic conditions were as follows: octadecylsilane bonded silica as a filler;mobile phase of acetonitrile-1%triethylamine solution (pH=3.0, ajusted withphosphoric acid)(26:74); detection wavelength was269nm; flow rate was1.0 mL/min; column temperature was35℃. Results indicated that under the testconditions, once detection and separation processes can be completed within20min,dialogue flexor separation and detection of food ingredients sanguinarine andchelerythrine base related processes such as quality control has a certain significance.Experimental results showed that the extraction, purification and testing ofherbal ingredients on the microfluidic chips is simple and convenient, low reagentconsumption, high sensitivity, short analysis time-consuming, precision, recovery canreach the requirements of pharmaceutical analysis. Compared with HPLC, GC andother precision instruments, microfluidic chips are cheap, simple and low cost, easierto promote. Therefore, microfluidic chip analysis with these advantages, itsapplications will become increasingly widespread in pharmaceutical analysis. |
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