Effect And Possible Mechanism Of MiR-145on The Invasion And Migration Of HepG2Cells

ObjectiveThis study is to investgate the effect of miR-145on the invasion and migration ofHepG2cells and the possible mechanisim，in order to lay a foundation for the treatmentof liver cancer.MethodsHepG2cells were cultured in vitro and transfected with miR-145mimics.At the sametime we set up blank control group,transfection reagent group,and NC negative controlgroup.Bioinformatics software forecasted the potential target genes of miR-145.Theproliferation of the HepG2was evaluated by CCK-8assay. Invasion and cell migratingability were evaluated by transwell invasion assay and scratch assay.After HepG2cellswere transfected with miR-145mimics,the mRNAs of miR-145and MUC1weredetected through QRT-PCR and the protein expression of MUC1was detected throughwestern-blot analysis.Results1.CCK-8method showed that cells transfected with miR-145mimics revealed significantlower growth ability compared to blank control group, transfection reagent group,and NCnegative control group(P﹤0.05).2.After HepG2cells transfected with miR-145mimics for72h, transwell invasion assayshowed the number of invaded cells in miR-145mimics group was lesser than that inblank control group, transfection reagent group,and NC negative control group(P﹤0.05).3. Scratch assay showed that scratches within24h and72h,the HepG2cells’s migrationability in miR-145mimics group was significantly lower than blank control group, transfection reagent group,and NC negative control group(P﹤0.05).4.QRT-PCR indicated that after HepG2cells transfected with miR-145mimics for48h,the mRNAs of miR-145was obviously up-regulated(P﹤0.05), but the mRNAs of MUC1didn’t change significantly(P﹥0.05).5.Western-blot indicated that after HepG2cells transfected with miR-145mimics for48h, the protein expression of MUC1decreased significantly(P﹤0.05).Conclusions1.MiR-145negatively regulates the proliferation,invasion and migration potential of livercancer cells HepG2.2.MiR-145may inhibit the invasion and migration of liver cancer cells HepG2throughtargeting MUC1.