RTP Affects The Proliferation,Apoptosis,Migration And Invasion Of Liver Cancer Cells By Down-regulating The Expression Of MiR-151

Objective:Liver cancer had the characteristics of high morbidity,high mortality and high degree of malignancy.It had seriously threatened the safety of human life.Due to the multi-drug resistance of liver cancer,most patients were already in advanced stages when diagnosed,leading to reduced therapeutic effects.Effective antitumor drugs were of great significance for the treatment of liver cancer.RTP had anti-oxidative stress,anti-inflammatory and anti-tumor effects,but its therapeutic effect on liver cancer cells and its mechanism of action had not yet been elucidated.This study mainly explores the effects of RTP on the proliferation,migration,invasion and apoptosis of liver cancer cells,and explores its possible mechanism of action.Methods:HepG2 cells were cultured in vitro and treated with RTP containing 0.65,1.25 and 2.5mg / mL RTP for 24 h.MTT method was used to detect cell proliferation.Flow cytometry was used to detect the apoptotic rate.Transwell chamber experiments were used to detect cell migration and invasion.qRT-PCR was used to detect miR-151 expression in cells.Anti-miR-NC and anti-miR-151 were transfected into HepG2 cells,respectively.miR-NC and miR-151 mimics were transfected into HepG2 cells,respectively,and treated with a medium containing RTP at a concentration of 1.25 mg / mL for 24 h.Cell proliferation anddecay were measured by MTT method,flow cytometry,and Transwell chamber experiments.Death,migration and invasion.Western blot was used to detect the expression of Cyclin D1,P21,Bax,E-cadherin,Bcl-2,and MMP-2 proteins.Results:Compared with the control group,the activity of HepG2 cells in the RTP 0.65 mg /mL group,RTP 1.25 mg / mL group,and RTP 2.5 mg / mL group was significantly reduced(P <0.05),and the level of Cyclin D1 protein was significantly reduced(P <0.05),the level of P21 protein was increased significantly(P <0.05).Compared with the control group,the apoptosis rate of HepG2 cells in the RTP 1.25 mg / mL group was significantly increased(P <0.05),the number of migrating and invading cells was significantly reduced(P <0.05),and the levels of Bax and E-cadherin protein were significantly increased(P <0.05),the levels of Bcl-2 and MMP-2 protein were significantly reduced(P <0.05).Compared with the control group,the expression level of miR-151 in HepG2 cells in the RTP 1.25 mg / mL group was significantly reduced(P <0.05).Compared with the anti-miR-NC group,the cell viability of the anti-miR-151 group was significantly reduced(P <0.05),and the apoptosis rate was significantly increased(P <0.05),the levels of Cyclin D1,Bcl-2,and MMP-2protein were significantly reduced(P <0.05),and the levels of P21,Bax,and E-cadherin protein were significantly increased(P <0.05).Compared with the RTP 1.25 mg / mL +miR-NC group,the cell viability of the RTP 1.25 mg / mL + miR-151 group was significantly increased(P <0.05),the number of migrating and invading cells was significantly increased(P <0.05),and the rate of apoptosis Significantly reduced(P <0.05),the levels of Cyclin D1,Bcl-2,and MMP-2 protein were significantly increased(P <0.05),and the levels of P21,Bax,and E-cadherin protein were significantly reduced(P <0.05).Conclusion:RTP can inhibit the proliferation,migration and invasion of liver cancer cells,and induce apoptosis,and its mechanism may be related to down-regulating the expression ofmiR-151.