The Research Of Gelatin Particles AggregationLess-Sensitive Method Utilized To Detect Human Immunodeficiency Virus Type-1Antibody On Gingival Crevicular Fluid And Oral Mucosal Transudate

[Objective] The aim of this study was to evaluate the feasibility of Gelatin particles Aggregation Less-Sensitive (PA-LS) method to detect HIV-1Antibody on Gingival Crevicular Fluid (GCF) and Oral Mucosal Transudate (OMT). The result of this study would be a foundation for non-invasive HIV infection test in dental clinic.[Methods] A total of1116participants, including50people whose HIV-1antibody positive,666drug users and400general people, who were used to gather the GCF and OMT samples, and the serum samples were gathered from the drug users simultaneously. And then, the GCF and OMT samples were detected by PA-LS, and the serum samples were screened by ELISA. For50people whose HIV-1antibody positive, the GCF or OMT samples were detected as HIV-1antibody negative or suspicious would be screened by ELISA and confirmed by WB on the serum samples. For666drug users, the GCF, OMT or serum samples were detected as HIV-1antibody positive or suspicious would be confirmed by WB on the serum samples. For400general people, the GCF or OMT samples were detected as HIV-1antibody positive or suspicious would be screened by ELISA and confirmed by WB on the serum samples. When got the final result which confirmed by WB, we calculated the sensitivity, the specificity, the omission diagnosic rate, the mistake diagnosic rate, positive predictive value and negative predictive value. Furthermore, Kappa statistics and chi-square tests would be used to carriy on the statistics.[Results](1) In this study, for those50people whose HIV-1antibody positive, the sensitivity of PA-LS detected HIV-1antibody positive on GCF samples was100%, the omission diagnostic rate was0; The sensitivity of PA-LS detected HFV-1antibody positive on OMT samples was100%, the omission diagnostic rate was0.(2) For666drug users, the sensitivity of PA-LS detected HIV-1antibody positive on GCF samples was100%, the specificity was99.10%, the omission diagnostic rate was0, the mistake diagnostic rate was0.90%, the positive predictive value was95.57%and the negative predictive value was100%; Contrast with ELISA detected HIV-1antibody positive on serum samples, the consistency of PA-LS detected HIV-1antibody positive on GCF samples was excellent(Kappa>0.8), the difference of PA-LS detected HIV-1antibody positive on GCF samples wasn’t significant(.P>0.05). The sensitivity of PA-LS detected HIV-1antibody positive on OMT samples was100%, the specificity was97.49%, the omission diagnostic rate was0, mistake diagnostic rate was2.51%, the positive predictive value was88.52%and the negative predictive value was100%; Contrast with ELISA detected HIV-1antibody positive on serum samples, the consistency of PA-LS detected HIV-1antibody positive on OMT samples was excellent(Kappa>0.8), the difference of PA-LS detected HIV-1antibody positive on OMT samples was significant (P>0.05),(3) For400people general people, all GCF samples showed HIV-1antibody negative; One OMT sample showed HIV-1antibody suspicious, and this OMT sample be redetected by PA-LS showed negative.[Conclusion] In this study, contrast with ELISA detected HIV-1antibody on serum samples, PA-LS detected HFV-1antibody on GCF samples was non-invasive and accurate; PA-LS detected HIV-1antibody on OMT samples was non-invasive but the accuracy of OMT samples was lower than GCF samples.