The Expression And Significance Of β-tubulin In Rat Brain Following Traumatic Brain Injury

Traumatic brain injury is a commonly disease in neurosurgery. The mortality,disability rate is at the top level of all kinds of traumatic injury. The complications suchas epilepsy, cognitive dysfunction seriously affect the quality of life and survival qualityof patients. As the lack of complete understanding of the biochemical changes of TBIafter the nerve cells, the selection of targeted therapy has been a difficult problem. Thetheory, method of treatment, curative effects is still no breakthrough progress. A largenumber of studies show that microtubulin protein family closely related not only with thecell division, proliferation, apoptosis, but also with the many nervous system diseasesand cognitive function. β-tubulin widely expressed in the cytoplasm of many kinds ofcells in tissue and cells, with tissue and cell specificity differentiation. β-tubulin is highlyexpressed in brain tissue. But there is no research reported the relation between neuroninjury and cognitive function. The author tries to explore the changes of β-tubulin proteinafter TBI through the rat TBI model.Objective: research on the mechanism of nerve injury in traumatic brain injury, tounderstand the relationship between β-tubulin and TBI and its expression changes afterTBI. TBI rat model simulated moderate TBI is established to investigate the mechanismof protein changes after TBI, and to provide the valuable theory basis to the protection ofbrain after TBI.Method: seventy male SD rats were divided randomly into normal control group (n=10)and TBI group (n=60). The TBI model was created by using lateral head rotation deviceand subdivided into6h,12h,24h,72h,7d and14d group (10rats per group). Theexpression and distribution of β-tubulin in the rat brain was investigatedimmunohistochemically. The expression of the brown stained particles was considered as "positive" and lack of the stained as "negative". To estimate the contribution of thenon-specific interaction binding, a negative antibody control is been used in each serialsections. The data was described by±SD, used the u test. The statistical tools:Statistical Package for the Social Sciences, SPSS17.0Result: β-tubulin was distributed on Cytochylema, axon and cytodendrite both in thehippocampus and the cortex. The expression of β-tubulin was decreased to the lowestlevel in the rat brain at6h post TBI (P <0.05), then became stronger gradually andrecovered to normal at day14, with no statistical difference compared with normalcontrol group (P>0.05).Conclusion: The lowest level expression of β-tubulin after TBI indicates that β-tubulinmay play an important role in the development of brain injury under effect of differentmechanisms. The mechanisms of the process of the repairment of the nerve injury inwhich β-tubulin take part need to be discovered, and so as to the drug intervention.