The Research On The New Electrodes Of Enzymetic Biofuel Cells

Enzymatic biofuel cell (EBFC) is one kind of device which could directly convert the chemical energy into electrical energy using mainly the enzyme extracted from the biological system as catalyst, and has advantages such as extensive fuel sources, moderate reaction conditions, high energy conversion rate and good biocompatibility. The research of EBFC focuses on finding new method to immobilize enzyme and developing new catalysts. The main works in this thesis are as follows:(1)Based on the mesoporous molecular sieve MCM-41in combination with carbon nanotubes (CNTs), the immobilization of2,2’amino-2(3-ethyl-benzo thiazole moiety sulfonic acid-6) ammonium salt (ABTS) and laccase was achieved. Energy disperse X-ray spectroscopy (EDS) and N2adsorption-desorption isotherms were used to characterize MCM-41. The electrochemical performance of the related enzymatic electrode was investigated by cyclic voltammetry (CV) and linear sweep voltammetry (LSV). The results are as follows:a large current of oxygen reduction (6.66μA) is achieved on the laccase-based elelctrode modified by MCM-41. The stability of ABTS immobilized on the electrode and the reusing property of laccase-based electrode are improved.(2) We developed a nitrogen-doped porous carbon nanocatalysts(PANI-C) as a non-platinum electrocatalysts for oxygen reduction. Scan electron microsopy (SEM) were employed to characterize the morphology of the catalysts. The electrocatalytic properties of the catalysts for oxygen reduction have been investigated by Electrochemical measurement methods (such as CV, LSV and chronoamperometric measurements) on rotating disk electrode. The PANI-C catalysts show a best long-term stability and tolerance to crossover effect towards oxygen reduction compared with the commercial Pt-C catalysts.(3)The thiol-β-cyclodextrin functionalized Halloysite nanotubes (HNT) was used to support Au nanoparticles and to immobilize Fc, and then to immobilize GOD by physical adsorption method. Thermogravimetry (TG) and transmission electron microscopy (TEM) were used to characterize the functionalized HNT. The electrochemical performance of the obtaind electrode was investigated by CV and LSV. The results are as follows:Ferrocene and glucose oxidase can be immobilize on HNT and exhibit good stability. For the assembled glucose/02fuel cell,, the open-circuit voltage was0.430V and the maximum power density at the voltage of0.308V was14.14μW·cm-2.