Preparation And Performance Of Enzymatic Biofuel Cell

Enzymatic biofuel cell is a device converting chemical energy directly with the biocatalysts, which has the advantage of abundant fuel resource, mild reaction condition and good biology consistency. In enzymatic biofuel cell aspect, more attention is concentrated on new method to immobilized enzyme. The details of preparation methods of enzymatic electrodes are especially described. This paper researched into the preparation and performance of single-compartmentalized and membrane electrode assembly enzymatic biofuel cells.The polymer films have three dimension configurations, which supply much available energy field. There are a lot of active groups in the polymer films. The polymer films have electrochemical catalyze characteristics. Because of its easy preparation and strong attachment to the electrode surface, the polymer modified electrode has distinguished itself as one of the most widely used chemically modified electrodes. This work prepared polymer chemically modified electrode, which acted as electron medium of immobilized enzyme electrode.The main research work is as follows:1. First of all, we prepared poly-(methylene green) chemically modified electrode by cyclic voltammetric method. We studied the PMG/CME of electropolymerization process, characterization, electrochemical behavior and electrocatalyzing NADH. The experiment result is PMG/CME has obvious electrocatalysis to NADH.2. We report the assembly of single-compartmentalized enzymatic biofuel cell. The alcohol dehydrogenase (ADH) and NAD~+ was immobilized in the PMG/CME by co-electrodepositing method as bioanode for the oxidation of alcohol. We used tradition method Pt/C catalyzer as cathode, oxygeon as cathodic fuel. Experiment indicated that the maximum open circuit voltage could reach 735 mV. The maximum output power density was 62.4μW/cm~2, output potential was about 395 mV at an external optimal load of 3 kΩand enzyme lifetime was two months.3. We designed a three-layer structural enzymatic electrode. The first layer is PMG and NAD~+, the second layer is PMG, the third layer is ion adsorption ADH. Then we fabricated membrane electrode assembly enzymatic biofuel cell. We also used tradition method Pt/C catalyzer as cathode, oxygeon as cathodic fuel. Also we measured the performance of membrane electrode assembly enzymatic biofuel cell. Experiment indicated that the maximum current density was 170±4.3μA/cm~2 and the maximum output power density was 72.3μW/cm~2 and the output potential was about 425 mV at an external optimal load of 300Ω. The maximum open circuit voltage could reach 630 mV. Dehydrogenase enzymes immobilized in the poly-methylene green membrane have shown high catalytic activity and can promote the direct bioelectrocatalysis for alcohol's oxidation.