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Preparation And Application Of Enzyme Electrode Modified With Carbon Nanotubes And Study Of The Enzymatic Biofuel Cell

Posted on:2009-09-07Degree:MasterType:Thesis
Country:ChinaCandidate:J Y LiFull Text:PDF
GTID:2132360248954430Subject:Applied Chemistry
Abstract/Summary:PDF Full Text Request
This thesis summarized two parts of the orginal work: part one is the preparation and application in the biofuel cell of glucose oxidase immboilized on functional multiwall carbon nanotubes. Part two is the preparation and research in performance of two kinds of biofuel cell based on alcohol dehydrogenas as catalyzer. One kind of biofuel cell is the dehydrogenase enzyme was immobilized on the carbon cloth by co-electrodepositing with the poly (methylene green) film, the other is dehydrogenase enzyme immobilized on the poly (methylene green) film on the carbon,which as the bioanode, and the cathode and proton-exchange membrane were pressed together,which like a membrane electrode.The main research work is as follows:1. We report the fabrication of a novel enzyme electrode that glucose oxidase immboilized on functional multiwall carbon nanotubes. Mesne production was studied by FI-RT and SEM and AFM and XRD. The adsorbance in enzyme of carbon nanotubes before and after a chemical treatment was contrasted, and the stability against temperature of free glucose oxidase was compared with the glucose oxidase immobilized on carbon nanotubes. What's more, we studied the electrochemistry characteristic of the enzyme electrode, the result proved glucose oxidase immboilized on functional multiwall carbon nanotubes had a bioelectrocatalysis on the oxidation of glucose, and it had a good stability against temperature.2. The above enzyme electrode as anode and graphite board with a serie of treatment make up of biofuel cell. The mechanism and performance of the biofuel cell were studied. It was found that the enzyme electrode modified with carbon nanoparticles could promote the direct bioelectrocatalysis for glucose oxidation and reduction of oxygen. This work provides a convenient method toward the preparation of biofuel cell.3.The dehydrogenase enzyme was immobilized on the carbon cloth by co-electrodepositing,when the poly (methylene green) film was forming. Thus we prepared anode and then use carbon with Pt as cathode to form a new kind biofuel cell baed on ethanol. The performance of the biofuel cell was measured and the principle of operation was studied.4. We fabricated a novel biofuel cell like a membrane electrode, which is the bioanode, and the cathode and proton-exchange membrane were pressed together, thereinto, the carbon cloth as basic electrode and poly (methylene green) film as carrier of alcohol dehydrogenase. We also studied the chemical performance and the mechanism of the biofuel cell.
Keywords/Search Tags:functionalized multiwall carbon nanotubes (MWCNTs), glucose oxidase, alcohol dehydrogenase, biofuel cells
PDF Full Text Request
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